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Titanium-enriched medium in shear-stressed endothelial cells: look on cell adhesion, survival and angiogenesis

Grant number: 19/09140-6
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): July 01, 2019
Effective date (End): January 19, 2020
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:Willian Fernando Zambuzzi
Grantee:Bruna Rodrigues Martins
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated research grant:14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration, AP.JP


Biomaterials have been the focus of research in the field of health, mainly because of their increasing importance for improving quality and human longevity. Although already is well established, the molecular and cellular effect of titanium is not yet fully understood. Our research group has worked extensively in the attempt to understand osteoblast adhesion on biomaterials. However, peri-implant healing and repair events require a recapitulation of cellular and molecular events, and endothelial cells are important in this scenario, and the shear stress model must be considered. As angiogenesis does not occur, in principle, juxtaposed to an implant surface, it is a rational process is an indirect effect within the surrounding tissue. To address this issue, we shall incubate titanium-based implants into cell culture mediums up to 24 hours, and later this medium will be used to treat endothelial cells up to 24 hours when the biological samples will be harvested to 1. Quantify titanium and aluminum without conditioned conditioning through a graphite furnace; 2. Investigation of the biological behavior of endothelial cells, by checking Integrin, FAK, Src, and Cofilin; 3. selection of response responses to treatment with titanium - Akt, MAPKs, VEGF and VEGFR; 4. Effect of conditioned medium on in vitro angiogenesis events.