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Bioluminescence of fungi, millipede and marine organisms: chemical and biological aspects

Grant number: 19/12605-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): July 01, 2019
Effective date (End): June 30, 2021
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Cassius Vinicius Stevani
Grantee:Douglas Moraes Mendel Soares
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:17/22501-2 - Electronic chemiexcitation in biological systems: bioluminescence and photochemistry in the dark, AP.TEM

Abstract

The present postdoctoral research project is based on the project submitted and approved by FAPESP 2017/22501-2. As mentioned in the proposal and thanks to Dr. Douglas's profile, he will be able to participate on the goals 5 and 6 of the proposal. More specifically in Goal 5 the postdoc can participate: (i) in the isolation and quantification of the biosynthetic intermediates of the various luciferin production routes, which will allow us to confirm the route of bioluminescent degradation and possible enzymatic recycling of luciferins, strongly suggested in the case of fungi; (ii) in the cloning of the enzymes Luz, HispS, H3H and CPH of diverse fungi and also to determine their activity and other enzymatic parameters; and (iii) in the study of the circadian regulation of the clock genes and the genes luz, hisps, h3h and cph; as well as about the evolution and phylogeny of these genes. In Goal 6, the researcher will assist: in field observation, collection, maintenance and laboratory breeding/cultivation of target bioluminescent organisms of this project: fungi, millipedes and polychaetes; in the purification and identification of luciferins and luciferins derivatives of the studied organisms, those already known and those that will be discovered; in the detailed mechanistic study of bioluminescence of fungi; in the investigation of mechanisms of light emission in millipedes and polychaetes; and in the determination of quantum yields, enzymatic parameters and free energy correlations. Goals 5 and 6 also involve extracting RNA and DNA to obtain transcriptomes and genomes, preparing cDNA libraries, conducting cloning experiments and purification of proteins of the organisms under study.