Heterologous expression and characterization of xilanases that belong to GH10 and GH11 families and evaluation of its application in xilan cleavage leading to xylooligossacharides production from lignocelulosic biomass

• Grant number: 19/10942-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2019
• End date: December 31, 2020
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Igor Polikarpov
• Grantee: Caio Cesar de Mello Capetti
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated research grant: 15/13684-0 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation, AP.TEM

Abstract

The greatest fraction of hemicellulose of plants is represented by xylan, which, after cellulose, is the most abundant natural and renewable polissacharide in nature. Hemicellulases, the enzymes capable of clevating beta 1,4 linkage that keeps together the monomers of xylose, building the main chain of xylan, are divided in many glycoside hydrolase families, the main ones being GH10 and GH11. Enzymatic degradation of xylan may produce monomers of xylose as well as oligossacharides (xylooligossacharides, XOs). XOs are considered to be pre-biotics, as they promote selective growth of beneficial microorganisms that make up the intestinal flora, such as those of the genus Bifidobacterium, responsible for many good effects on human health. In this project, we propose making biochemical and biophysical characterizations of two xylanases, from families GH10 and GH11, as well as its use in xylan degradation and XOS production from lignocellulosic biomass. The biochemical and biophysical properties of the enzymes, and the products obtained will be compared, which is convenient because xylanases with different properties are important to produce xylooligossacharides. This could grant us products with different degrees of polimeriation and ramification.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAPETTI, CAIO CESAR DE MELLO; PELLEGRINI, VANESSA OLIVEIRA ARNOLDI; SANTO, MELISSA CRISTINA ESPIRITO; CORTEZ, ANELYSE ABREU; FALVO, MAURICIO; CURVELO, ANTONIO APRIGIO DA SILVA; CAMPOS, ELEONORA; FILGUEIRAS, JEFFERSON GONSALVES; GUIMARAES, FRANCISCO EDUARDO GONTIJO; DE AZEVEDO, EDUARDO RIBEIRO; et al. Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies. Carbohydrate Polymers, v. 299, p. 12-pg., 2023-01-01. (19/10942-0, 15/13684-0)