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E. coli as platform for L-aspartate production from hemicellulose hydrolysates

Grant number: 20/12428-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2020
Effective date (End): September 30, 2021
Field of knowledge:Engineering - Chemical Engineering
Principal Investigator:Roberto de Campos Giordano
Grantee:Rafael de Paula
Home Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
Associated research grant:16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales, AP.BIOEN.TEM

Abstract

Lignocellulosic biomass has been considered as an alternative source of energy in view of the growing concern with the development of more sustainable production processes. Thus, new technologies are being developed for the use of these agro-industrial residues as a fermentative substrate to produce compounds with high added value. From the pre-treated lignocellulosic biomass, sugars like glucose or xylose can be readily fermented, but according to the type of pre-treatment of the material, a significant fraction of C5 sugars can remain in the form of xylooligosaccharides (XOS), which are not metabolized by some important microorganisms in industrial bioprocesses, such as the bacterium Escherichia coli. Thus, to enable a better use of lignocellulosic materials for fermentative purposes with the consumption of all available sugar fractions, we propose in this project the implementation of the route of consumption of xylooligosaccharides in Escherichia coli through the heterologous expression of the xylanolytic system present in Bacillus subtilis 168. It is expected that the implementation of this route of XOS consumption will improve cell growth, and consequently also improve the productivity of metabolites of interest in the context of biorefineries. In future works, the production of L-aspartate using hydrolysates from the hydrothermal treatment of sugarcane bagasse will be used as a case study to evaluate the constructed strain.