Effect of intrauterine ozone therapy on endometrial angiogenesis of non-pregnant mares

• Grant number: 19/11450-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2019
• End date: July 31, 2020
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: Jair Camargo Ferreira
• Grantee: Letícia Mara Cardoso
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

The present study will describe, for the first time, the effect of ozone (O3) therapy on endometrial angiogenesis of non-pregnant mares. A total of 21 females will be assigned into one of four groups according to the treatment: O3, ½O3 and Control groups (n = 7 mares/group). Mares from O3, ½O3 and Control groups will receive intrauterine treatment with ozone-oxygen gas mixture containing 42ug, 21ug and 0ug O3/mL, respectively. The treatments will be performed every three days and during six days. In each treatment, a flow of 0.25 mL gas/min will be infused during three consecutive minutes. Endometrial fragments and inflammatory cells will be collected immediately before the first treatment and 24 hours after the third insufflation by uterine biopsy and endometrial cytology, respectively. The histological analysis of the uterine angiogenesis will be based on the number of endometrial blood vessels, the degree of endometrial vascularization and the vascularized endometrial area. In addition, six endometrial morphological structures will be photodocumented for posterior digital analysis: a) Diameter of blood vessels, b) intraglandular secretion, c) luminal epithelium height, d) glandular epithelium height, e) glandular diameter, and f) glandular lumen.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, JAIR CAMARGO; AMARAL, RAFAEL SILVA; CARDOSO, LETICIA MARA; MARCHIO, SOPHIA PANELLI; RODRIGUES, MARCELA ALDROVANI; FONSECA ALVES, CARLOS EDUARDO; DE MEIRA, CEZINANDE. Endometrial angiogenesis induced by uterine insufflation with an oxygen-ozone gas mixture in mares. REPRODUCTION IN DOMESTIC ANIMALS, JUN 2021. (19/11450-3, 19/22728-2, 19/11680-9)

FERREIRA, JAIR CAMARGO; AMARAL, RAFAEL SILVA; CARDOSO, LETICIA MARA; MARCHIO, SOPHIA PANELLI; RODRIGUES, MARCELA ALDROVANI; FONSECA ALVES, CARLOS EDUARDO; DE MEIRA, CEZINANDE. Endometrial angiogenesis induced by uterine insufflation with an oxygen-ozone gas mixture in mares. REPRODUCTION IN DOMESTIC ANIMALS, v. 56, n. 9, p. 1176-1183, SEP 2021. (19/11680-9, 19/11450-3, 19/22728-2)