Analysis and the expression of Sirt1 and its modulation for resveratrol in vitro model of Pré-Eclampsia

• Grant number: 19/06118-0
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: August 01, 2019
• End date: June 30, 2020
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Valeria Cristina Sandrim
• Grantee: Sarah Viana Mattioli
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

In Brazil and many other countries pre eclampsia (PE) stands out as the main hypertensive disorder in pregnancy, being potentially fatal for both mother and embryo and affecting 3-8% of all pregnancies in the world. However, little is known about the etiology of this pathology. It is believed that utero-placental ischemia causes the release of placental factors in the maternal circulation, which end up causing damage to the endothelium of these pregnant women through alterations in gene expression, alteration un the production of vasoactive factors and damage by oxidative stress. SIRT1 is a class III protein of histone deacetylases that exhibit NAD + dependent function. Being highly expressed in endothelial cells, this protein is one of the main molecules to combat oxidative stress in the organism, besides regulating gene expression and the release of nitric oxide (NO) by eNOS. In order to evaluate the relationship between PE and SIRT1 in vitro, human umbilical vein endothelial cells (HUVECs) will be incubated with plasma of healthy pregnant women, hypertensive pregnant women and pre-eclamptic pregnant women in the presence or absence of Resveratrol, a potent natural antioxidant and activator of SIRT1, and EX-527, an inhibitor of SIRT1 activity. Therefore, the expression and activity of SIRT1, besides its possible effects on endothelial damage, reactive oxygen species (ROS) and nitric oxide (NO), will be analyzed.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIANA-MATTIOLI, SARAH; CINEGAGLIA, NAIARA; BERTOZZI-MATHEUS, MARIANA; BUENO-PEREIRA, THAINA OMIA; CALDEIRA-DIAS, MAYARA; CAVALLI, RICARDO CARVALHO; SANDRIM, VALERIA CRISTINA. SIRT1-dependent effects of resveratrol and grape juice in an in vitro model of preeclampsia. BIOMEDICINE & PHARMACOTHERAPY, v. 131, NOV 2020. (19/06118-0, 19/07230-8, 15/20461-8)

VIANA-MATTIOLI, SARAH; NUNES, PRISCILA; CAVALLI, RICARDO; SANDRIM, VALERIA. Analysis of SIRT1 Expression in Plasma and in an In Vitro Model of Preeclampsia. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, v. 2020, APR 28 2020. (08/53593-0, 19/06118-0, 15/20461-8)

VIANA-MATTIOLI, SARAH; BUENO-PEREIRA, THAINA OMIA; SANDRIM, VALERIA CRISTINA. Letter to the editor regarding: ``Resveratrol and endothelial function: A literature review{''}. PHARMACOLOGICAL RESEARCH, v. 172, OCT 2021. (19/26640-2, 19/07230-8, 19/06118-0)