Paper-Based Electrochemical Biosensor for Traumatic Brain Injury detection

• Grant number: 19/00166-2
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): August 01, 2019
• Effective date (End): September 30, 2024
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Lauro Tatsuo Kubota
• Grantee: Patrícia Batista Deroco
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis, AP.TEM
• Associated scholarship(s): 21/14732-0 - Development of electrochemical devices based on graphene obtained by advanced inkjet-printing and laser-scribing techniques as platforms to monitor biomarkers, BE.EP.PD

Abstract

Traumatic brain injury (TBI) consider as a "silent epidemic," is a worldwide, common but disregarded disease. Only in the USA every year almost one and a half million people have been affected by TBI, which causes serious disabilities and frequently leads to death. It cost is estimated at 4 billion dollars annually and its the cause of socio-economic issues as it is touching mostly young and professionally active people. There is an evident lack of a proper method suitable for TBI diagnosis especially in case of the mild type known as a concussion. Therefore, a fast, small and handheld detection system is required to accelerate the patient diagnosis and avoid severe complications. In this context, the design of novel pump-free electrochemical paper-based analytic device for sensitive and reproducible traumatic brain injury detection at serum and cerebrospinal fluid is proposed in this project. Paper-based screen-printed biosensors, modified with conductive nanostructures like carbon and metal nanoparticles, as well as, polymer dendrimers, will be fabricated using a precise ink-jet method. This approach allows decreasing the cost and significantly simplifies the invention. After this step, receptors compatible with TBI biomarkers will be immobilized on surface sensor for ensure the high specificity and reproducibility. Following the fabrication and characterization steps by spectroscopic and microscopic methods, the proposed electrode architectures will be applied for the single or multi-markers electrochemical detection in the same device. We hope to achieve a reliable device for detecting the severity of TBI for on-site patient diagnostics in every environment. It will increase the quality of many people life and bring a positive socio-economic effect.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

WACHHOLZ JUNIOR, DAGWIN; DEROCO, PATRICIA BATISTA; KUBOTA, LAURO TATSUO. A copper-based metal-organic framework/reduced graphene oxide-modified electrode for electrochemical detection of paraquat. Microchimica Acta, v. 189, n. 8, p. 12-pg., 2022-08-01. (21/09706-0, 19/00166-2, 13/22127-2, 14/50867-3)

DEROCO, PATRICIA BATISTA; WACHHOLZ JUNIOR, DAGWIN; KUBOTA, LAURO TATSUO. Recent advances in point-of-care biosensors for the diagnosis of neglected tropical diseases. SENSORS AND ACTUATORS B-CHEMICAL, v. 349, DEC 15 2021. (14/50867-3, 13/22127-2, 19/00166-2)

DEROCO, PATRICIA BATISTA; WACHHOLZ JUNIOR, DAGWIN; KUBOTA, LAURO TATSUO. Paper-based Wearable Electrochemical Sensors: a New Generation of Analytical Devices. Electroanalysis, v. N/A, p. 12-pg., 2022-06-28. (21/14732-0, 14/50867-3, 13/22127-2, 19/00166-2, 21/09706-0)