Novel cellular scaffold from a histidine rich recombinant elastin-derived protein for 3D printing

• Grant number: 19/10789-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: September 01, 2019
• End date: April 30, 2021
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Luiz Henrique Catalani
• Grantee: Michelle da Silva Liberato
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine, AP.TEM

Abstract

Currently, molecular biology applied to the production of recombinant proteins has proven to be highly effective in biomedicine. In this context, the elastin-like polypeptides (ELPs) are among the most promising stimulation-responsive biopolymers to be expressed genetically. Moreover, the proper expression system depends on the protein produced. In this case, the ELP-40 will be expressed by using the technology of recombinant DNA in lineages of the bacteria Escherichia coli (E. coli). Particularly, it is proposed the development of frameworks produced by 3D printing. For such, we suggest the inclusion of Histidine as a key amino acid in the ELP-40 sequence, targeting the generation of bioactive hydrogels highly elastic and sensitive to photocrosslinking by singlet oxygen. These biomaterials are highly attractive as structural support, aiming the cellular growth, as well as adhesion, proliferation, and differentiation; therefore, they present important uses in the area of regenerative medicine, including its use as inks for 3D printing techniques based on stereolitography. Finally, the scaffolds will be characterized by advanced experimental techniques, so to infer over its structural and morphological properties, besides biological trials for the evaluation of biocompatibility through tests in vitro and in vivo.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZELENOVSKII, PAVEL S.; ROMANYUK, KONSTANTIN; LIBERATO, MICHELLE S.; BRANDAO, PAULA; FERREIRA, FABIO F.; KOPYL, SVITLANA; MAFRA, LUIS M.; ALVES, WENDEL A.; KHOLKIN, ANDREI L.. 2D Layered Dipeptide Crystals for Piezoelectric Applications. ADVANCED FUNCTIONAL MATERIALS, v. 31, n. 43, JUL 2021. (19/10789-7, 14/50867-3)

LIBERATO, MICHELLE S.; CAVALCANTE, NAYARA G. S.; SINDU, P. ABINAYA; RODRIGUES-JESUS, MONICA J.; ZELENOVSKII, PAVEL; CARREIRA, ANA C. O.; BAPTISTA, MAURICIO S.; SOGAYAR, MARI C.; FERREIRA, LUIS C. S.; CATALANI, LUIZ H.. Histidine-based hydrogels via singlet-oxygen photooxidation. SOFT MATTER, v. 17, n. 48, NOV 2021. (18/13492-2, 19/08975-7, 19/10789-7)