Metabolomic analysis by Nuclear Magnetic Resonance of blood samples from individuals submitted to cardiorespiratory rehabilitation.

• Grant number: 19/10756-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: September 01, 2019
• End date: August 31, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Antonio Gilberto Ferreira
• Grantee: Roberta Manzano Maria
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated research grant: 16/22215-7 - Impact of inspiratory muscle training and aging on metabolic mapping, autonomic modulation, and cardiovascular, respiratory and metabolic responses, and prediction of cardiorespiratory health through wearables, AP.TEM

Abstract

Introduction: NMR has been shown to be a very useful analytical tool, especially in the last decade, in the metabolomics/metabonomics study of animal and plant organisms. Its great advantage when not looking for specific substances (non targed compounds) lies in the very few steps to prepare the sample and, mainly, in the reproducibility of the experiments, in such a way that a chemometric analysis of the results becomes feasible. In addition, the results are quantitative compared to the results obtained by coupled high efficiency liquid chromatography techniques using several targed compounds. On the other hand, the positive results generated by the use of inspiratory muscle training (IMT) in active and non-active healthy individuals have made this type of intervention one of the most studied in the last decades. IMT has already been used as an integral part of cardiorespiratory rehabilitation in individuals with respiratory muscle weakness and currently appears as an alternative to improve the athletic performance of amateur and elite athletes by reducing the sensation of dyspnea in these individuals. In addition, regular physical activity is one of the main tools for healthy aging, so the metabolic characterization of active and sedentary aging can elucidate metabolic changes during the aging process.