Photo- and termo-reception systems in the Blue Crab, Callinectes sapidus (Crustacea Brachyura), and their relationship with the molt cycle

• Grant number: 18/23043-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: September 01, 2019
• End date: November 30, 2022
• Field of knowledge: Biological Sciences - Physiology - Compared Physiology
• Principal Investigator: Ana Maria de Lauro Castrucci
• Grantee: Daniela Dantas David
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Environmental signals synchronize endogenous rhythms to external clues, mainly light-dark and temperature cycles, through clock genes transcription and translation along 24 hours. The clock machinery is present in the mammalian central oscillator and in peripheral tissues of all vertebrates studied to date. In crustaceans, a central oscillator has not been yet identified, and photoreceptors containing long- and short-wavelength opsins are distributed throughout the central nervous system. In addition to being light sensors, opsins such as the mammalian melanopsin and Drosophila rhodopsin, which share the same signaling pathway, were also characterized as thermoreceptors. These photopigments ultimately trigger the opening of Transient Receptor Potential channels (TRP) and membrane depolarization. TRP channels may directly respond to stimuli, such as temperature. In crustaceans a few have been sequenced, however their functionality remains unknown. In addition to light and temperature, hormonal factors from X-Organ/Sinus Gland (XO/SG) complex in association with Y-organ, also regulate rhythmic functions such as the molt cycle. Having this in mind, we propose to determine, along the molt cycle of Callinectes sapidus: 1) the influence of photoperiod and temperature on clock genes, opsins and TRP channels in XO/SG complex, Y-organ, cerebral ganglion and hepatopancreas in vivo; 2) the autonomy of photo- and thermo-reception of these organs and the role of opsins and TRP channels in vitro. For that, our experimental approaches will be quantitative PCR for clock genes, opsins, TRP channels and molt-inhibiting hormone (CasMIH); immunohistochemistry of the hepatopancreas; quantification of ecdysteroids and melatonin by ELISA and radioimmunoassay, and serotonin by HPLC in vivo and in organ explants of Callinectes sapidus subject to 8:16 LD, 12:12 LD and 16:8 LD, combined with 17°C, 22°C and 27°C. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SUA-CESPEDES, CRISTHIAN; LACERDA, JOSE THALLES; ZANETTI, GIOVANNA; DAVID, DANIELA DANTAS; MORAES, MARIA NATHALIA; DE ASSIS, LEONARDO V. M.; CASTRUCCI, ANA MARIA L.. Melanopsin (OPN4) is a novel player in skin homeostasis and attenuates UVA-induced effects. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 242, p. 15-pg., 2023-04-03. (16/00696-3, 13/07937-8, 19/19005-9, 21/01659-2, 17/26651-9, 18/23043-0, 18/16511-8, 12/50214-4, 12/12663-1, 18/14728-0, 17/24615-5, 20/04524-8, 14/16412-9)

SUA-CESPEDES, CRISTHIAN D.; DAVID, DANIELA DANTAS; SOUTO-NETO, JOSE A.; LIMA, OTONIEL GONCALVES; MORAES, MARIA NATHALIA; DE ASSIS, LEONARDO V. MONTEIRO; CASTRUCCI, ANA MARIA DE LAURO. Low Temperature Effect on the Endocrine and Circadian Systems of Adult Danio rerio. FRONTIERS IN PHYSIOLOGY, v. 12, NOV 24 2021. (18/23043-0, 18/16511-8, 17/26651-9, 18/14728-0, 17/24615-5, 18/23915-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

DAVID, Daniela Dantas. Effect of temperature on neuroendocrine aspects of the molt cycle in the blue crab Callinectes sapidus (BRACHYURA:PORTUNIDAE). 2023. Doctoral Thesis - Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB) São Paulo.