Histological and molecular evaluation of the effects of direct and remote ischemic postconditioning on acute intestinal ischemia in young rats

• Grant number: 19/12465-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2019
• End date: March 31, 2021
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Ana Cristina Aoun Tannuri
• Grantee: Mateus Souza Abreu
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Acute mesenteric ischemia (AMI) is a vascular emergency requiring early diagnosis and intervention to prevent intestinal necrosis and death. AMI is classically characterized by poorly specific clinical, laboratory and radiological findings, which results in diagnostic difficulty contributing to high lethality (60-80%). In addition, it is a relevant complication of several conditions that affect the neonatal and pediatric age group. Attempts to treat AMI through tissue reperfusion face the challenge of phenomenon of ischemia-reperfusion injury (I/R), in which restoration of blood flow after the original ischemic event aggravates tissue damage. Ischemic postconditioning (IPC) was developed in order to minimize the damage caused by revascularization. Many authors have demonstrated the effectiveness of IPC as a way to reduce I/R damage in AMI in adult rats. However, there are no equivalent studies on this relation in young rat models (more suitable for assessing possible clinical applications in the pediatric and neonatal population).In this study, the direct and indirect IPC techniques will be compared with I/R, as well as the two IPC techniques wilo be compared with each other, in the context of AMI in young animal models (21-day-old Wistar rats and 50-80 grams). Thirty-two animals will be allocated into the groups: control, ischemia and reperfusion injury, direct ischemic post-conditioning and remote ischemic post-conditioning. Biopsies will be performed for histological and molecular analysis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MATEUS SOUZA ABREU; ANA CRISTINA AOUN TANNURI; RAFAEL FELIPE GONÇALVES RODRIGUES; RAFAEL JOSÉ DA SILVA; JOSIANE DE OLIVEIRA GONÇALVES; SUELLEN SERAFINI; UENIS TANNURI. Effects of local and remote ischemic postconditioning methods on ischemiareperfusion injury in a young animal model of acute mesenteric ischemia. Acta Cirurgica Brasileira, v. 38, 2023-06-02. (19/12465-4)

ABREU, MATEUS SOUZA; TANNURI, ANA CRISTINA AOUN; RODRIGUES, RAFAEL FELIPE GONCALVES; DA SILVA, RAFAEL JOSE; GONCALVES, JOSIANE DE OLIVEIRA; SERAFINI, SUELLEN; TANNURI, UENIS. Effects of local and remote ischemic postconditioning methods on ischemia-reperfusion injury in a young animal model of acute mesenteric ischemia. Acta Cirurgica Brasileira, v. 38, p. 10-pg., 2023-01-01. (19/12465-4)