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Histopathological, immunohistochemical, and endocrinological effects of cocaine in marine fish

Grant number: 19/20187-4
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): January 08, 2020
Effective date (End): January 07, 2021
Field of knowledge:Physical Sciences and Mathematics - Oceanography - Biological Oceanography
Principal Investigator:Camilo Dias Seabra Pereira
Grantee:Mayana Karoline Fontes
Supervisor abroad: Anna Capaldo
Home Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
Local de pesquisa : Università degli Studi di Napoli Frederico II (UNINA), Italy  
Associated to the scholarship:16/24033-3 - Ecotoxicological study and environmental risk assessment of Cocaína in marine ecosystems, BP.DR

Abstract

Residues of cocaine (COC) have been identified in different aquatic ecosystems. Once in the environment, COC could interact with non-target organisms. Several studies showed that environmentally concentrations of COC induced negative effects in invertebrate organism, especially freshwater, but there are still few studies on the effects of COC on marine vertebrates. The aim of this study is to investigate the histopathological, immunohistochemical, and endocrinological effects of COC in the European eels. The fish (30 adult specimens) will be acclimatized for one month and expose to nominal concentration of 200 ng.L-1 of COC (corresponding to the concentration found in Santos Bay, SP, Brazil, by Fontes et al. 2019). At the end of the experiment, all eels will be anesthetized and from each animal the gonads will be removed for the evaluation of morphology (histology), localization of enzymes involved in the steroidogenesis pathway (immunohistochemistry) and sex steroid levels as testosterone (DiaMetra), dihydrotestosterone (Abnova) and 17²-estradiol (DiaMetra) (endocrinological effects). The data obtained will provide an effective diagnosis of the environmental quality associated with COC, enabling the qualitative and quantitative identification of its impacts on marine ecosystems.