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Fat transplantation as a strategy to restore the fertility of BTBR ob/ob mice and increase the pool of animals in the vivarium

Grant number: 19/12636-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): November 01, 2019
Effective date (End): October 31, 2020
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Érika Bevilaqua Rangel
Grantee:Maria Theresa Araújo Balby Rocha
Home Institution: Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE). Sociedade Beneficente Israelita Brasileira Albert Einstein (SBIBAE). São Paulo , SP, Brazil


The BTBR ob/ob mice (black and tan, obese, tufted) are characterized by a mutation on the leptin gene (Lep ob ) that makes it knockout for this gene. Due to this mutation, BTBR mice show a number of characteristics, such as:hyperfagia, hyperglycemia, glucose intolerance, hyperisulinism,hypertriglyceridemia, impaired healing, albuminuria, hypothermia, andincreased production of pituitary and adrenal hormones. Moreover, theseanimals show a secondary subfertility related to hypogonadotrophichypogonadism because leptin has a direct effect on the central nervous systemthrough the release of the GnRH hormones and, subsequently, the release ofthe luteinizing hormone (LH) and the follicle stimulating hormone (FSH). Severalstrategies were tested with the aim of repairing the fertility of the ob/ob mice,including restricted diets and treatment with recombinant human and mouseleptin. Furthermore, previous literature showed that the fat transplantation was able to repair the fertility of the ob mice, decrease its body weight and increase the blood plasma concentration of leptin. However, most of these articles did not specify how much fat and time is necessary for the transplantation to work. Therefore, it is expected that specifying these methods will increase the pool of BTR ob/ob mice in the laboratory vivarium.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SAVIO-SILVA, CHRISTIAN; BEYERSTEDT, STEPHANY; SOINSKI-SOUSA, POLIANA E.; CASARO, EXPEDITO B.; BALBY-ROCHA, MARIA THERESA A.; SIMPLICIO-FILHO, ANTONIO; ALVES-SILVA, JAMILLE; RANGEL, ERIKA B. Mesenchymal Stem Cell Therapy for Diabetic Kidney Disease: A Review of the Studies Using Syngeneic, Autologous, Allogeneic, and Xenogeneic Cells. STEM CELLS INTERNATIONAL, v. 2020, NOV 20 2020. Web of Science Citations: 0.

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