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Electronic device for the diagnosis of Human Visceral Leishmaniasis

Grant number: 19/25277-1
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): February 01, 2020
Effective date (End): October 31, 2022
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
Principal Investigator:Wendel Andrade Alves
Grantee:Freddy Alejandro Nunez Esteves
Home Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
Associated research grant:17/02317-2 - Interfaces in materials: electronic, magnetic, structural and transport properties, AP.TEM

Abstract

Leishmaniasis is parasitic diseases caused by different protozoan species of the genus Leishmania and transmitted by sand flies. This disease has limited treatments and in many cases, has unwanted effects, which makes prior diagnosis essential. The most common and definitive diagnostic method is the optical observation of the parasite through culture in a specific medium. Despite its excellent specificity, it is a dangerous and time-consuming procedure, as errors can lead to fatalities. As an alternative to optical diagnosis, other possibilities can be explored, such as kDNA identification by PCR and detection of antigens/antibodies in blood samples through assays such as ELISA and IFAT. In this proposal, an architecture already described in the literature by the research group (ACS Appl. Electron. Mater. 2019, 1, 2086-2095), in partnership with Rheabiotech Ltda, will be optimized for field detection. For this, portable potentiostat measurements will be validated, followed by simplification of data acquisition and analysis. Finally, a database will be built using sera from different healthy individuals and patients with Visceral and Cutaneous Leishmaniasis for cross-validation of a binary classification model (healthy or infected) by using logistic regression curves (ROC). Also, transistorized devices for Leishmaniasis detection will be constructed. For this, other peptides will be synthesized due to their performances demonstrated in previous works of the group. (AU)