Effect of incorporation of cellulose nanocrystals on adhesion and biofilm formation of simple and mixed Candida albicans and methicillin-sensitive Staphylococcus aureus (MSSA) in a denture base resin

• Grant number: 19/07491-6
• Support Opportunities: Scholarships in Brazil - Master
• Start date: March 01, 2020
• End date: April 30, 2021
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Ana Carolina Pero
• Grantee: Thais de Sousa Santana
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The use of biopolymers from renewable sources as nanometric reinforcing agents can represent an important innovation in dental materials. The aim of this study will be evaluate the simple and mixed biofilm formation of Candida albicans and methicillin-susceptible Staphylococcus aureus (MSSA), on the surface of acrylic resin for denture base, after the incorporation of cellulose nanocrystals (CNC). Cellulose nanocrystals (CNC) isolated from wood pulp will be incorporated into acrylic resin for denture base (Onda-Cryl - OC, activated by microwave energy). Disc-shaped specimens (15 x 3 mm) will be obtained for each condition (groups 0% - control, and 0,75%) Prior to the adhesion and biofilm formation tests, the surface roughness will be measured in a digital rugosimeter Ra (¼m) and the surface free energy will be measured from contact angle measurements, obtained by means of a goniometer connected to a computerized system. The formation of biofilms will be performed from the inoculation of C.albicans and S.aureus in acrylic resin discs incubated at 37ºC for 90 minutes and 48h. After this period, the analysis of cell metabolism test (XTT) will be performed; evaluation of antimicrobial activity by counting colony-forming units (CFU/ml) and analysis by confocal laser microscopy. All analyses will be performed in triplicate on 3 distinct occasions for 90 minutes and 48 hours periods. Data will be tabulated and submitted to adequate statistical tests, according to adherence to normality, at 5% of significance level. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANTANA, THAIS DE SOUSA; LEITE, ANDRESSA ROSA PERIN; PASQUINI, DANIEL; PERO, ANA CAROLINA. ffect of the incorporation of cellulose nanocrystals into a microwave-polymerized denture base resin: Cellulose nanocrystals into a denture base resi. STARCH-STÄRKE, v. 74, n. 3-4, NOV 2021. (17/26512-9, 19/07491-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SANTANA, Thais de Sousa. Effect of incorporation of cellulose nanocrystals in a denture base resin on superficial properties and simple and mixed Candida albicans and Methicillin-sensitive Staphylococcus aureus (MSSA) biofilms. 2021. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.