Determination of molecular and cellular mechanisms associated with Sepsis outcome using single cell RNA sequencing

• Grant number: 19/15070-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2020
• End date: February 28, 2023
• Field of knowledge: Biological Sciences - Immunology - Cellular Immunology
• Principal Investigator: Fernando de Queiroz Cunha
• Grantee: Guilherme Cesar Martelossi Cebinelli
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 13/08216-2 - CRID - Center for Research in Inflammatory Diseases, AP.CEPID

Abstract

Sepsis is defined as a life-threatening organ dysfunction that is caused by a dysregulated host response to infection. There is estimate an annual occurrence of 31.5 million cases of Sepsis and 19.4 million cases of septic shock in the world, that potentially causing 5.3 million deaths. Knowing these alarming indications in 2017, the World Health Organization (WHO) adopted a resolution aimed at improving the prevention, diagnosis and treatment of this neglected clinical condition. In this context, new therapies, such as immunotherapies, have been tested in Sepsis; but with no success. One possible explanation is that the therapeutic targets used for the development of these immunotherapies were not adequate, suggesting that the pathophysiology of Sepsis is not fully understood. Thus, more specific molecular studies may contribute to the identification of new immunotherapeutic targets in Sepsis. In this context, our work has an innovative proposal and have the aim to identify new markers by analyzing the transcriptional profile of the different leukocyte subtypes comparing surviving and non-surviving septic animals those have significant lesions in vital organs, using the technology of large scale analysis for individual cells, the single cell RNA sequencing. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVES DAMASCENO, LUIS EDUARDO; MARTELOSSI CEBINELLI, GUILHERME CESAR; FERNANDES, MARIANE FONT; NASCIMENTO, DANIELE CARVALHO; PUBLIO, GABRIEL AZEVEDO; RAMIREZ VINOLO, MARCO AURELIO; OLIVEIRA, SERGIO COSTA; SPARWASSER, TIM; CUNHA, THIAGO MATTAR; CUNHA, FERNANDO QUEIROZ; et al. STING is an intrinsic checkpoint inhibitor that restrains the T(H)17 cell pathogenic program. CELL REPORTS, v. 39, n. 8, p. 15-pg., 2022-05-24. (19/15070-0, 20/04170-1, 18/17542-4, 13/08216-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

CEBINELLI, Guilherme Cesar Martelossi. Determination of the molecular and cellular mechanisms related to the immune response associated with the susceptibility of isogenic mice to sepsis. 2023. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina de Ribeirão Preto (PCARP/BC) Ribeirão Preto.