Study of the miRNAs profile of boars ejaculates with different freezability

• Grant number: 18/12500-1
• Support Opportunities: Scholarships in Brazil - Master
• Start date: February 01, 2020
• End date: February 28, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Reproduction
• Principal Investigator: André Furugen Cesar de Andrade
• Grantee: Ana Carolina Pedrosa
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Pig industry must always cherish the interactivity and globalization of its information, and the generated researches should reach the greatest number of farms as possible, so that knowledge is widely disseminated. Genetically superior semen diffusion is facilitated by the use of frozen semen. However, the freezing results obtained from the breeding centers are differents and demonstrate variable boar performances. Several laboratory analyzes aim to understand more clearly the predisposing causes for these results. There is still no definitive protocol for cryopreservation of boar semen and, while research advances on this scenario, in parallel there are molecular studies being developed about micro-RNAs transcribed in spermatozoa, which can regulate gene expression and control much of this process in animal organisms. Micro-RNA can be differentially expressed during spermatogenesis, ejaculation and cryopreservation, but there is still a lack of data to support the understanding of behavior and action of these molecules in pig andrology. In order to contribute to literature data about the regulation of gene expression by micro-RNAs, the present experiment aims to determine the micro-RNA profile in seminal plasma and in raw boar spermatozoa and compare the results with complementary laboratory analysis to classify the different boar with regard to cryotolerance of their spermatozoa. Further understanding of this process may elect the profile of micro-RNAs as a boar congelability biomarker, an important additional tool for genetically diffusing farms. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEDROSA, ANA CAROLINA; TORRES, MARIANA ANDRADE; ALKMIN, DIEGO VILELA; PINZON, JORGE E. P.; MASSAMI KITAMURA MARTINS, SIMONE MARIA; DA SILVEIRA, JULIANO COELHO; CESAR DE ANDRADE, ANDRE FURUGEN. Spermatozoa and seminal plasma small extracellular vesicles miRNAs as biomarkers of boar semen cryotolerance. Theriogenology, v. 174, p. 60-72, OCT 15 2021. (16/24690-4, 18/12500-1, 15/21829-9, 14/22887-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PEDROSA, Ana Carolina. Study of miRNAs profile of boars\' ejaculates with different freezability. 2021. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) São Paulo.