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Study of the efficiency and safety of the vibratory platform as an adjuvant in equine training

Grant number: 19/16779-3
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2020
Effective date (End): February 28, 2023
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal Investigator:Guilherme de Camargo Ferraz
Grantee:Júlia Ribeiro Garcia de Carvalho
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The Vibratory Platforms (VP) are devices capable of generating oscillations, being considered a form of passive exercise. Several benefits of using vibrating platforms have been described, such as increased muscle strength, improved physical performance and improved balance and functional mobility. VPs have been studied and used in many human applications, such as part of a physiotherapeutic and non-pharmacological protocol, or as an alternative or supplementary method to exercise. However, there are few animal studies, including the equine species. The present study will investigate the effects of using the vibratory platform as an adjuvant in equine training. For this, nine equine animals will be used, mixed-breed, males and females, with average age of 12 years and average weight of 450 kg. Three experimental groups will be performed: Control Group (CG), which will train only on the automatic walker; vibratory platform group (GPV), which will be submitted to warm-up, training and warm-down in VP; and trained group (GTPV), who will be submitted to training in the automatic walker associated with sessions of warm-up and warm-down in the VP. The experiment will be performed in the cross-over scheme, with training duration of 4 weeks and wash out of at least 30 days. After the end of each training period, the animals will be submitted to the Incremental Exercise Test (IET) on a high performance treadmill to evaluate physiological changes and physical fitness. The heart rate will be monitored during the IET. Blood samples will be taken to determine lactate and glucose before the start of the experiment, 7, 14, 21 and 28 days after the start of each training and during the IET. Blood samples will also be taken to determine creatine kinase before, 7, 14, 21 and 28 days after the start of each training and 6 and 24 hours after the end of each IET. In addition, biopsies of the gluteus medius muscle will be performed prior to the training, and immediately and 6 hours after each IET, to evaluate muscle glycogen, muscle lactate, monocarboxylate transporters and muscle typing by immunohistochemical method. Thermographic evaluations will also be performed before the start of the experiment, 7, 14, 21, and 28 days after the start of each training. Statistical analysis will be done using the one-way ANOVA test, which will be applied to compare variables between more than two groups. For the intra-group comparison, the paired Student t-test will be used. Values of p <0.05 will be considered significant. All analyzes will be performed in the Sigma Plot 12.0 program. (AU)