Determination of mechanical properties of polysaccharide-based magnetic scaffolds

• Grant number: 20/01907-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: June 01, 2020
• End date: September 30, 2023
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Denise Freitas Siqueira Petri
• Grantee: Alex Carvalho Alavarse
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/13492-2 - Synthetic and natural scaffolds applied to regenerative medicine, AP.TEM
• Associated scholarship(s): 22/08442-1 - 3D hydrogel with tunable rheological properties for in vitro cancer studies, BE.EP.PD

Abstract

In this project, magnetic materials will be created through in situ synthesis of magnetite nanoparticles (MNP), using polysaccharides (alginate, xanthan, chitosan) matrices or through the incorporation of MNP in the polysaccharide matrices. The mechanical properties of magnetic hydrogels will be evaluated according to the MNP concentration, ionic strength of medium and polysaccharides charge density, in the absence and presence of External Magnetic Field (EMF). The mechanical properties of magnetic hydrogels will be determined on a nanometric nanoscale by means of atomic force microscopy (AFM), in the absence and presence of EMF. The mechanical properties will be correlated with proliferation and differentiation of neuronal cells. The adhesion properties between cells and polysaccharide-coated microspheres (colloidal probes) will be evaluated by means of force measurements in AFM. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALAVARSE, ALEX CARVALHO; FRACHINI, EMILLI CAROLINE GARCIA; DA SILVA, RAFAEL LEONARDO CRUZ GOMES; LIMA, VITORIA HASHIMOTO; SHAVANDI, AMIN; PETRI, DENISE FREITAS SIQUEIRA. Crosslinkers for polysaccharides and proteins: Synthesis conditions, mechanisms, and crosslinking efficiency, a review. International Journal of Biological Macromolecules, v. 202, p. 39-pg., 2022-01-25. (20/03248-7, 20/01907-3, 19/22671-0, 20/05632-9, 18/13492-2)

ALAVARSE, ALEX CARVALHO; SILVA, JEAN BEZERRA; ULRICH, HENNING; SIQUEIRA PETRI, DENISE FREITAS. Poly(vinyl alcohol)/sodium alginate/magnetite composites: magnetic force microscopy for tracking magnetic domains. SOFT MATTER, v. 19, n. 14, p. 11-pg., 2023-03-15. (18/13492-2, 18/07366-4, 20/01907-3, 19/08178-0)

ALAVARSE, ALEX CARVALHO; FRACHINI, EMILLI CAROLINE G.; SILVA, JEAN BEZERRA; PEREIRA, RAFAEL DOS SANTOS; ULRICH, HENNING; SIQUEIRA PETRI, DENISE FREITAS. Amino acid decorated xanthan gum coatings: Molecular arrangement and cell adhesion. CARBOHYDRATE POLYMER TECHNOLOGIES AND APPLICATIONS, v. 4, p. 11-pg., 2022-06-13. (20/01907-3, 18/07366-4, 18/13492-2)