Physical exercise as a non-pharmacological strategy in the prevention and treatment of Alzheimer's Disease

• Grant number: 19/17532-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: June 01, 2020
• End date: March 31, 2024
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Adelino Sanchez Ramos da Silva
• Grantee: Gustavo Paroschi Morais
• Host Institution: Escola de Educação Física e Esporte de Ribeirão Preto (EEFERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The number of people with Dementia is increasing substantially around the world, having a global cost similar to GDP of countries like Netherlands. Brazil occupies the ninth place in this ranking. Alzheimer Disease (AD) is the most prevalent among the neurodegenerative diseases (Dementias), having the formation of ²-amiloide plaques in the brain tissue as a characteristic. One of the factors involved in the ²-amiloide malformation and aggregation is the impairment in the autophagic-lysosomal pathway, decreasing ²-amiloide removal, allowing its aggregation, leading to neurodegeneration. Since its discovery, TFEB has demonstrated a fundamental role in the autophagic-lysosomal pathway. TFEB is the major autophagic coordinator, positively regulating lysosomal biogenesis and autolysosome formation, having the capability to ameliorate the pathological condition in neurodegenerative disease. Physical exercise is an effective approach to prevent neurodegeneration, however, the mechanisms of protection need better elucidation. Physical exercise was capable to improve neural autophagy and decrease neurodegeneration through TFEB, and in skeletal muscle through Calcineurin-TFEB pathway activation, induce autophagy. Furthermore, physical exercise was capable to induce mitochondrial biogenesis, and improve its function in skeletal muscle in a TFEB dependent manner. It is known that mitochondrial disfunctions and neurodegenerative disease have a close correlation, and mitochondrial autophagy has an important role in this condition. Therefore, the main objective of the present study is to verify if TFEB, through the autophagic-lysosomal and mitochondrial biogenesis pathways, along with Calcineurin, would be exerting its neuroprotector effect induced by the physical exercise. Mice will be divided into 4 groups: control (CT; sedentary), trained (TR; submitted to exercise protocol), sedentary AD (SAD; APP/PS1 sedentary), trained AD (TAD; APP/PS1 submitted to exercise protocol). The methods include: immunoblotting, Real-time PCR, immunofluorenscense and brain imaging. Level of significance of p<0.05 will be adopted. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA ROCHA, ALISSON L.; PINTO, ANA P.; BEDO, BRUNO L. S.; MORAIS, GUSTAVO P.; OLIVEIRA, LUCIANA C.; CAROLINO, RUITHER O. G.; PAULI, JOSE R.; SIMABUCO, FERNANDO M.; DE MOURA, LEANDRO P.; ROPELLE, EDUARDO R.; et al. Exercise alters the circadian rhythm of REV-ERB-alpha and downregulates autophagy-related genes in peripheral and central tissues. SCIENTIFIC REPORTS, v. 12, n. 1, p. 13-pg., 2022-11-21. (21/08693-1, 19/17532-1, 17/25492-4, 19/11820-5, 17/19869-8, 17/12765-2)

OLIVEIRA, LUCIANA DA COSTA; MORAIS, GUSTAVO PAROSCHI; DE OLIVEIRA, FRANCIANE PEREIRA; MATA, MILENE MONTAVONI; VERAS, ALLICE SANTOS CRUZ; DA ROCHA, ALISSON LUIZ; ELIAS, LUCILA LEICO KAGOHARA; TEIXEIRA, GIOVANA RAMPAZZO; DE MORAES, CAMILA; CINTRA, DENNYS E.; et al. Intermittent fasting combined with exercise training reduces body mass and alleviates hypothalamic disorders induced by high-fat diet intake. JOURNAL OF NUTRITIONAL BIOCHEMISTRY, v. 119, p. 12-pg., 2023-05-29. (16/17968-6, 20/08192-0, 19/17532-1, 17/25492-4, 19/21709-4, 21/06291-3)