Mechanisms related to dental protection against tooth erosion by treatment with a new sugar cane-derived cystatin (CaneCPI-5)

• Grant number: 20/02263-2
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• Effective date (Start): December 01, 2020
• Effective date (End): May 31, 2021
• Field of knowledge: Health Sciences - Dentistry - Social and Preventive Dentistry
• Principal Investigator: Marília Afonso Rabelo Buzalaf
• Grantee: João Victor Frazão Câmara
• Supervisor: Matthias Hannig
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Research place: Saarland University, Homburg, Germany
• Associated to the scholarship: 18/24450-9 - Evaluation of effect of a new cystatin derived from sugar cane on the profile and viability of microcosm biofilm, as well and on dentin demineralization prevention, BP.MS

Abstract

The acquired pellicle (AP) is a film that results from selective adsorption of proteins, glycoproteins and lipids on the tooth surface. The AP forms a protective interface on the tooth surface that reduces the demineralizing effects of acids. Recently, a new cystatin derived from sugarcane (CaneCPI-5) was shown to strongly bind to enamel and protect against initial enamel erosion in vitro. However, the exact mechanisms by which the protection occurs are not known so far. Thus, the aim of this study will be to provide additional insights into the mechanisms of dental protection by treatment with CaneCPI-5, by evaluating the thickness, viscoelastic properties and morphology of the AP formed on the tooth surface after treatment with this protein, as well as the amount of CaneCPI-5 adsorbed in the AP layer. (AU)