Chromosomal evolution, genetic diversity and demographic history in species of the genus Pyrrhulina (Characiformes, Lebiasinidae)

• Grant number: 19/25045-3
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: June 01, 2020
• End date: August 29, 2024
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: Marcelo de Bello Cioffi
• Grantee: Renata Luiza Rosa de Moraes
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated scholarship(s): 22/04964-3 - Satellitome analysis in Pyrrhulina species (Characiformes, Lebiasinidae): insights for karyotype and sex chromosome evolution, BE.EP.DD

Abstract

Although fishes have traditionally been the subject of comparative evolutionary studies, the Lebiasinidae family has long been little cytogenetically explored, possibly because of difficulties in obtaining good chromosomal preparations due to the small size of its representatives. However, this scenario has presented significant changes due to the initial contributions of the proponent of this project made during its scientific initiation, from conventional and molecular cytogenetic analyzes performed in four Pyrrhulina species. The results showed a large karyotype variation present in the group accompanied by significant changes in the amount of heterochromatin, multigene families' distribution pattern and in the presence of a multiple sex chromosome system in only one of the analyzed species. Thus, the present project intends to analyze the chromosomal and genomic diversification that occurred in the genus Pyrrhulina, which will focus on comparative chromosomal and genomic analyzes in other species of the genus, focusing on the processes of karyotypic evolution that occurred in this fish group. In addition, the genetic distances of the examined species will be evaluated by genotyping using next generation sequencing (DArTseq). The data will also allow a broad investigation into the influence of the development of multiple sex chromosome systems on genetic diversity, reduction of gene flow and consequently on the speciation process. Finally, the results will provide a clear picture of the genetic diversity present among species and demographic models that may explain basin colonization will be elaborated, clarifying the current geographical distribution of this group. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROSA DE MORAES, RENATA LUIZA; CAVALCANTE SASSI, FRANCISCO DE MENEZES; FERREIRA MARINHO, MANOELA MARIA; RAB, PETR; REBELO PORTO, JORGE IVAN; FELDBERG, ELIANA; CIOFFI, MARCELO DE BELLO. Small Body, Large Chromosomes: Centric Fusions Shaped the Karyotype of the Amazonian Miniature Fish Nannostomus anduzei (Characiformes, Lebiasinidae). GENES, v. 14, n. 1, p. 12-pg., 2023-01-01. (20/11772-8, 20/02681-9, 19/25045-3)

ROSA DE MORAES, RENATA LUIZA; CAVALCANTE SASSI, FRANCISCO DE MENEZES; CARLOS BERTOLLO, LUIZ ANTONIO; FERREIRA MARINHO, MANOELA MARIA; VIANA, PATRIK FERREIRA; FELDBERG, ELIANA; SALES OLIVEIRA, VANESSA CRISTINA; DEON, GEIZE APARECIDA; AL-RIKABI, AHMED B. H.; LIEHR, THOMAS; et al. Tracking the Evolutionary Trends Among Small-Size Fishes of the Genus Pyrrhulina (Characiforme, Lebiasinidae): New Insights From a Molecular Cytogenetic Perspective. FRONTIERS IN GENETICS, v. 12, OCT 6 2021. (20/11772-8, 20/02681-9, 19/25045-3)

FERREIRA, PEDRO H. N.; SOUZA, FERNANDO H. S.; DE MORAES, RENATA L.; PEREZ, MANOLO F.; SASSI, FRANCISCO DE M. C.; VIANA, PATRIK F.; FELDBERG, ELIANA; EZAZ, TARIQ; LIEHR, THOMAS; BERTOLLO, LUIZ A. C.; et al. The Genetic Differentiation of Pyrrhulina (Teleostei, Characiformes) Species is Likely Influenced by Both Geographical Distribution and Chromosomal Rearrangements. FRONTIERS IN GENETICS, v. 13, p. 9-pg., 2022-05-04. (20/11772-8, 19/25045-3)