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Development of a simple and affordable disposable device for rapid diagnosis of Coronavirus (SARS-CoV-2)

Grant number: 20/07539-6
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): June 01, 2020
Effective date (End): March 31, 2022
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal Investigator:Ronaldo Censi Faria
Grantee:Tássia Regina de Oliveira
Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
Associated research grant:20/04635-4 - Development of a simple and affordable disposable device for rapid diagnosis of coronavirus (SARS-CoV-2), AP.R

Abstract

With the recent pandemic caused by the new Coronavirus (SARS-CoV-2), an early diagnosis and isolation of infected patients have become essential measures to reduce and delay viral spread. However, the insufficient number of diagnostic tests for the infection has limited the ability of clinicians and public health specialists to accurately determine and track the prevalence, severity, mortality and transmissibility of this disease. In this project, it is proposed the development of a disposable, simple and low-cost microfluidic device for the detection of three RNA sequences of the SARS-CoV-2 virus N1 gene, aiming at the quick and accurate diagnosis of COVID-19. The performance of our research group has been guided by the development of devices like this for the detection of protein biomarkers aiming at the diagnosis of diseases with the partnership of research groups in the health area, which has generated important results with a strong impact on innovation. Furthermore, it is proposed to build a microfluidic platform using easily accessible materials and simple equipment, which will allow the analysis of different samples simultaneously. The device will consist of several microfluidic channels, each containing four independent sensor regions, which will allow detection by electrochemiluminescence of the three RNAs of the virus in addition to a human RNA, which will be used as the device's internal control. The applicability of the devices to be built will be evaluated initially in standard samples containing synthetic sequences (positive and negative) and, in a second moment, in samples from patients infected with SARS-CoV-2 supplied by the UFSCar University Hospital. The project also aims at training human resources at different levels and publishing articles in indexed national and international journals as well as filing patents. Therefore, the development of a device for rapid, selective and low-cost detection of viral RNA will allow the early diagnosis of SARS-CoV-2 infection, while meeting an international public health need. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NASCIMENTO, EVAIR D.; FONSECA, WILSON T.; DE OLIVEIRA, TASSIA R.; DE CORREIA, CAMILA R. S. T. B.; FACA, VITOR M.; DE MORAIS, BEATRIZ P.; SILVESTRINI, VIRGINIA C.; POTT-JUNIOR, HENRIQUE; TEIXEIRA, FELIPE R.; FARIA, RONALDO C.. COVID-19 diagnosis by SARS-CoV-2 Spike protein detection in saliva using an ultrasensitive magneto-assay based on disposable electrochemical sensor. SENSORS AND ACTUATORS B-CHEMICAL, v. 353, p. 9-pg., . (19/03943-0, 16/25798-3, 20/12920-0, 20/04635-4, 20/07539-6)
CATUNDA, LUCAS GOMES DA SILVA; DO PRADO, THIAGO MARTIMIANO; DE OLIVEIRA, TASSIA REGINA; DOS SANTOS, DANIEL JUNIOR ALMEIDA; GOMES, NATHALIA OEZAU; CORREA, DANIEL SOUZA; FARIA, RONALDO CENSI; MACHADO, SERGIO ANTONIO SPINOLA. SARS-CoV-2 detection enabled by a portable and label-free photoelectrochemical genosensor using graphitic carbon nitride and gold nanoparticles. Electrochimica Acta, v. 451, p. 10-pg., . (22/02164-0, 20/04635-4, 18/22214-6, 20/09587-8, 20/07539-6)
DE OLIVEIRA, TASSIA REGINA; LEITE, TAISE HELENA OLIVEIRA; MIRANDA, WYLLIAN NEVES; MANULI, ERIKA REGINA; LEAL, FABIO; SABINO, ESTER; POTT-JUNIOR, HENRIQUE; MELENDEZ, MATIAS; FARIA, RONALDO CENSI. Molecular test for COVID-19 diagnosis based on a colorimetric genomagnetic assay. Analytica Chimica Acta, v. 1257, p. 7-pg., . (20/04635-4, 20/07539-6)

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Filed patent(s) as a result of this research project

DISPOSITIVO ELETROQUÍMICO E MÉTODO PARA DETECÇÃO DA PROTEÍNA SPIKE DO VÍRUS SARS-CoV-2 EM SALIVA HUMANA PARA DIAGNÓSTICO DE COVID-19 BR1020210051256 - Fundação Universidade Federal de São Carlos (UFSCar) ; Universidade de São Paulo (USP). . RONALDO CENSI FARIA (UFSCar) ; FELIPE ROBERTI TEIXEIRA (UFSCar) ; WILSON TIAGO DA FONSECA (UFSCar) ; TÁSSIA REGINA DE OLIVEIRA (UFSCar) ; EVAIR DIAS NASCIMENTO (UFSCar) ; CAMILA ROLEMBERG SANTANA TRAVAGLINI BERTI DE CORREIA (UFSCar) ; VITOR MARCEL FAÇA (USP) ; BEATRIZ PEREIRA DE MORAIS (USP) ; VIRGINIA CAMPOS SILVESTRINI (USP) e HENRIQUE POTT JUNIOR (UFSCar). - March 2021, 18

MÉTODO GENOMAGNÉTICO E COLORIMÉTRICO PARA DETECÇÃO E QUANTIFICAÇÃO DE RNA DO VÍRUS SARS-CoV-2 BR1020210078820 - Clonning Solutions Pesquisa e Desenvolvimento LTDA. ; Fundação Universidade Federal de São Carlos (UFSCar) ; Universidade de São Paulo (USP) . RONALDO CENSI FARIA (UFSCar) ; HENRIQUE POTT JUNIOR (UFSCar) ; TÁSSIA REGINA DE OLIVEIRA (UFSCar) ; WYLLIAN NEVES MIRANDA (UFSCar) ; MATIAS ELISEO MELENDEZ (Clonning Solutions) ; TAISE HELENA OLIVEIRA LEITE (UFSCar) e ESTER CERDEIRA SABINO (USP). - April 2021, 26

SUPORTES MAGNÉTICOS PARA MAGNETO-ENSAIOS UTILIZANDO MICROPLACA DE 96 POÇOS BR1020210156465 - Fundação Universidade Federal de São Carlos (UFSCar) . RONALDO CENSI FARIA (UFSCar) ; TÁSSIA REGINA DE OLIVEIRA (UFSCar) e TAISE HELENA OLIVEIRA LEITE (UFSCar). - September 2021, 08

SISTEMA DE DETECÇÃO OU QUANTIFICAÇÃO DE RNA POR ELETROQUIMIOLUMINESCÊNCIA, USO DO SISTEMA E MÉTODO PARA DETECÇÃO DE RNA BR1020210184191 - Clonning Solutions Pesquisa e Desenvolvimento LTDA. ; Fundação Universidade Federal de São Carlos (UFSCar) ; Universidade de São Paulo (USP) ; Universidade Municipal de São Caetano do Sul (UCSC) . RONALDO CENSI FARIA (UFSCar) ; TÁSSIA REGINA DE OLIVEIRA (UFSCar) ; MATIAS ELISEO MELENDEZ (Clonning Solutions) ; TAISE HELENA OLIVEIRA LEITE (UFSCar) ; ESTER CERDEIRA SABINO (USP) ; HENRIQUE POTT JUNIOR (UFSCar) ; FABIO EUDES LEAL (UCSC) e ERIKA REGINA MANULI (UCSC). - September 2021, 16