Evaluation of the interaction of biofilms mono and dual-species of Candida spp and dermatophytes photodynamic therapy combined with 2-chalcone

• Grant number: 19/22188-8
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: June 01, 2020
• End date: January 31, 2022
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Maria José Soares Mendes Giannini
• Grantee: Níura Madalena Bila
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Dermatomycoses are mainly caused by fungi of the dermatophyte family and yeast species of the genera Candida. Dermatophytosis is a superficial infection of keratinized structures such as skin, hair and nails caused by filamentous fungi of the family Arthrodermataceae. The main genera are Microsporum, Epidermophyton, Trichophyton. Among the yeast, some species of the genus Candida are normal human microbiota and may become pathogenic in case of internal and external factors causing candidiasis, most common fungal disease in the world. Dermatophytes and Candida species can form biofilms increasing virulence, drug resistance and consequently persistence of infection. In clinical practice, there are reports of mixed infection between Candida and dermatophytes associated with superficial mycoses. Previous studies have shown an antagonistic interaction between T. rubrum and C. albicans/C. parapsilosis biofilms, with inhibition of T. rubrum growth but in contrast inhibition of Candida species filamentation especially C. albicans. To date was not discovered drug able to eradicate the fungal biofilms at therapeutic concentrations. Chalcones are natural plant products that have potential antimicrobial activities and photosensitizing properties. Photodynamic therapy is a promising modality because its effectiveness against a wide range of microorganism, as photodynamic inactivation may occur in sensitive or resistant strains to conventional drugs. Thus, this work intends to study the interaction of dermatophytes and Candida species, mainly C. albicans and C. parapsilosis, with verification of the factors that act on dual-species biofilms. On the other hand, to evaluate the antibiofilm capacity of 2-chalcone without and with the combination of photodynamic therapy in Candida and dermatophyte monoespécies and mixed biofilms, and to verify the mechanism of antifungal action of 2-chalcone. It is expected that to be identified the metabolite (s) capable (s) to act on the interaction between both fungi during biofilm condition and that 2-chalcone is a potent and inexpensive therapeutic alternative for the treatment of Dermatophytosis. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COSTA-ORLANDI, CAROLINE BARCELOS; MARTINEZ, LUIS R.; BILA, NIURA MADALENA; FRIEDMAN, JOEL M.; FRIEDMAN, ADAM J.; MENDES-GIANNINI, MARIA JOSE S.; NOSANCHUK, JOSHUA D.. Nitric Oxide-Releasing Nanoparticles Are Similar to Efinaconazole in Their Capacity to Eradicate Trichophyton rubrum Biofilms. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, JUL 15 2021. (18/02785-9, 17/18388-6, 19/22188-8)

VASO, CAROLINA ORLANDO; BILA, NIURA MADALENA; PANDOLFI, FABIANA; DE VITA, DANIELA; BORTOLAMI, MARTINA; CARVALHO BONATTI, JEAN LUCAS; DE MORAES SILVA, ROSANGELA APARECIDA; CARVALHO GONCALVES, LARISSA NAIARA; TUDINO, VALERIA; COSTI, ROBERTA; et al. Evaluation of the Anti-Histoplasma capsulatum Activity of Indole and Nitrofuran Derivatives and Their Pharmacological Safety in Three-Dimensional Cell Cultures. PHARMACEUTICS, v. 14, n. 5, p. 23-pg., 2022-05-01. (20/15586-4, 19/22188-8, 18/02785-9, 17/18388-6, 16/11836-0)

GARCIA, LETICIA MORAIS; COSTA-ORLANDI, CAROLINE BARCELOS; BILA, NIURA MADALENA; VASO, CAROLINA ORLANDO; GONCALVES, LARISSA NAIARA CARVALHO; FUSCO-ALMEIDA, ANA MARISA; MENDES-GIANNINI, MARIA JOSE SOARES. A Two-Way Road: Antagonistic Interaction Between Dual-Species Biofilms Formed byCandida albicans/Candida parapsilosisandTrichophyton rubrum. FRONTIERS IN MICROBIOLOGY, v. 11, SEP 4 2020. (18/02785-9, 17/18388-6, 19/22188-8)

BILA, NIURA MADALENA; COSTA-ORLANDI, CAROLINE BARCELOS; VASO, CAROLINA ORLANDO; BONATTI, JEAN LUCAS CARVALHO; DE ASSIS, LETICIA RIBEIRO; REGASINI, LUIS OCTAVIO; FONTANA, CARLA RAQUEL; FUSCO-ALMEIDA, ANA MARISA; MENDES-GIANNINI, MARIA JOSE SOARES. 2-Hydroxychalcone as a Potent Compound and Photosensitizer Against Dermatophyte Biofilms. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, MAY 13 2021. (18/02785-9, 19/22188-8, 20/15586-4, 17/18388-6)

COSTA-ORLANDI, CAROLINE B.; BILA, NIURA M.; BONATTI, JEAN LUCAS C.; VASO, CAROLINA O.; SANTOS, MARIANA B.; POLAQUINI, CARLOS R.; BIASIOLI, MARIANA M. SANTONI M.; HERCULANO, RONDINELLI D.; REGASINI, LUIS O.; FUSCO-ALMEIDA, ANA MARISA; et al. Membranolytic Activity Profile of Nonyl 3,4-Dihydroxybenzoate: A New Anti-Biofilm Compound for the Treatment of Dermatophytosis. PHARMACEUTICS, v. 15, n. 5, p. 19-pg., 2023-05-04. (17/18388-6, 19/22188-8, 18/02785-9, 20/15586-4)

VASO, CAROLINA ORLANDO; PANDOLFI, FABIANA; BILA, NIURA MADALENA; DE VITA, DANIELA; BORTOLAMI, MARTINA; SOARES MENDES-GIANNINI, MARIA JOSE; TUDINO, VALERIA; COSTI, ROBERTA; COSTA-ORLANDI, CAROLINE BARCELOS; FUSCO-ALMEIDA, ANA MARISA; et al. Synthesis and Evaluation of the Antifungal and Toxicological Activity of Nitrofuran Derivatives. PHARMACEUTICS, v. 14, n. 3, p. 19-pg., 2022-03-01. (20/15586-4, 16/11836-0, 19/22188-8, 17/18388-6)

GONCALVES, LARISSA NAIARA CARVALHO; COSTA-ORLANDI, CAROLINE BARCELOS; BILA, NIURA MADALENA; VASO, CAROLINA ORLANDO; DA SILVA, ROSANGELA APARECIDA MORAES; MENDES-GIANNINI, MARIA JOSE SOARES; TAYLOR, MARIA LUCIA; FUSCO-ALMEIDA, ANA MARISA. Biofilm Formation byHistoplasma capsulatumin Different Culture Media and Oxygen Atmospheres. FRONTIERS IN MICROBIOLOGY, v. 11, JUL 10 2020. (17/18388-6, 19/22188-8)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

BILA, Níura Madalena. Evaluation of the effect of 2-chalcone mediated by photodynamic therapy on mono- and dual-species biofilms of Candida spp. and dermatophytes and mechanisms of interaction of microorganisms in the mixed biofilm. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Farmacêuticas. Araraquara Araraquara.