Evaluation of the effectiveness of entomopathogenic bacteria and nematodes for the control of brown stink bug Euschistus heros

• Grant number: 20/04676-2
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2020
• End date: October 14, 2020
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Luís Garrigós Leite
• Grantee: Samanta Letícia Lopes Nanzer
• Host Institution: Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 17/50334-3 - Institutional research development plan (PDIp): modernization and adequacy of strategic multi-user units of the Instituto Biológico, AP.PDIP

Abstract

Phytophagous stink bug are the most important group of soybean pests in Brazil, as they feed directly on pods, reaching the grains. Euschistus heros, known as brown stink bug, is the most abundant among stink bug in this culture. Entomopathogenic nematodes (NEPs) have the ability to kill insects due to the host's search behavior in the soil and symbiotic associations with bacteria that cause pathogenesis. Different entomopathogenic bacteria have proven toxic activity in several insect families. This work aims to evaluate species of bacteria and entomopathogenic nematodes as to the potential for E. heros control. The tests will be divided into three stages: species selection; concentration test and substrate test. For the selection of bacterial isolates, the insects will be immersed in the bacterial suspension. For the selection of NEPs isolates, the nematodes will be inoculated in moist sand. The isolate that shows greater virulence in the previous tests will be selected for the concentration test. The concentrations will be defined according to the chosen isolate and the area of the plastic pot (113.04 cm²). Once the best concentration is selected, the substrate test will be performed. This concentration will be applied in the four treatments: (1) 200g sand; (2) 1000g sand; (3) straw; (4) 1000g sand + straw, also in plastic pots (1L). All experiments will consist on six repetitions, containing ten insects in each repetition, and a bean pod. Mortality assessment will be performed on the 2nd, 5th and 7th day. Therefore, it is intended to provide a promising isolate for the control of the brown stink bug. And knowledge about the concentrations and influence of the substrate in the isolate, aiming at better use of this organism against the pest. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOPES NANZER, SAMANTA LETICIA; RECCHIA, GUSTAVO HENRIQUE; CHACON-OROZCO, JULIE GIOVANNA; SATOCHI ABE SILVA, RAPHAEL; FRANCO MARINGOLI CARDOSO, JORGE; GARRIGOS LEITE, LUIS. Assessment of entomopathogenic nematodes and their symbiotic bacteria to control the stink bugs Euschistus heros and Dichelops melacanthus (Heteroptera: Pentatomidae) in the soybean-corn succession system. TURKISH JOURNAL OF ZOOLOGY, v. 45, n. 5, SI, p. 356-371, 2021. (20/04676-2)