Analysis of synaptosomes isolated from hippocampus tissue of patients with Mesial Temporal Lobe Epilepsy using proteomics and metabolomics

• Grant number: 19/25948-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: August 01, 2020
• End date: November 30, 2024
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Iscia Teresinha Lopes Cendes
• Grantee: Amanda Morato Do Canto
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 13/07559-3 - BRAINN - The Brazilian Institute of Neuroscience and Neurotechnology, AP.CEPID

Abstract

Epilepsy is a chronic neurological disorder characterized by the occurrence of epileptic seizures due to abnormal neuronal discharges. Mesial Temporal Lobe Epilepsy (MTLE) is the most common type of focal epilepsy and present a high proportion of patients who do not respond to treatment with Antiepileptic Drug Treatment (AED). Most of these patients with drug-resistant epilepsy show a type of hippocampal lesion characterized by extensive neuronal damage and atrophy, called Hippocampal Sclerosis (HS). The synapses are electric and chemical communications between the neurons and synaptosomes are membrane vesicles that contain the synaptic components. The study of synaptosomes can give clues about the synaptic transmission and its abnormalities, since they contain all the machinery involved in the release, reuptake and storage of neurotransmitters. In addition, synaptic proteins have been studied as possible therapeutic targets in many neurological diseases. In this context, we propose to analyze the synaptosomes isolated from brain tissue (hippocampus) of patients with MTLE in comparison with normal tissue, using proteomics and metabolomics to evaluate the changes in their molecular content which can be related to epileptogenesis and the mechanisms underlying AED-resistance. This information can also be used subsequently in the identification of new therapeutic targets for patients with drug resistant MTLE. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MAURER-MORELLI, CLAUDIA, V; DE VASCONCELLOS, JAIRA F.; BRUXEL, ESTELA M.; ROCHA, CRISTIANE S.; DO CANTO, AMANDA M.; TEDESCHI, HELDER; YASUDA, CLARISSA L.; CENDES, FERNANDO; LOPES-CENDES, ISCIA. Gene expression profile suggests different mechanisms underlying sporadic and familial mesial temporal lobe epilepsy. Experimental Biology and Medicine, v. 247, n. 24, p. 18-pg., 2022-10-19. (19/25948-3, 18/03254-7, 05/56578-4)

BRUXEL, ESTELA M.; BRUNO, DANIELLE C. F.; DO CANTO, AMANDA M.; GERALDIS, JAQUELINE C.; GODOI, ALEXANDRE B.; MARTIN, MARIANA; LOPES-CENDES, ISCIA. Multi-omics in mesial temporal lobe epilepsy with hippocampal sclerosis: Clues into the underlying mechanisms leading to disease. SEIZURE-EUROPEAN JOURNAL OF EPILEPSY, v. 90, n. SI, p. 34-50, AUG 2021. (20/06168-4, 19/25948-3, 18/03254-7, 19/20953-9, 19/00213-0, 17/26167-0)

BRUXEL, ESTELA M.; DO CANTO, AMANDA M.; BRUNO, DANIELLE C. F.; GERALDIS, JAQUELINE C.; LOPES-CENDES, ISCIA. Multi-omic strategies applied to the study of pharmacoresistance in mesial temporal lobe epilepsy. EPILEPSIA OPEN, OCT 2021. (19/25948-3, 20/06168-4, 13/07559-3, 18/03254-7, 17/26167-0)

DIANA MARCELA MEJÍA-GRANADOS; BENJAMÍN VILLASANA-SALAZAR; ANA CAROLINA COAN; LIARA RIZZI; MARCIO LUIZ FIGUEREDO BALTHAZAR; ALEXANDRE BARCIA DE GODOI; AMANDA MORATO DO CANTO; DOUGLAS CESCON DA ROSA; LUCAS SCÁRDUA SILVA; RAFAELLA DO ROSARIO TACLA; et al. O microbioma intestinal nas doenças neuropsiquiátricas. Arquivos de Neuro-Psiquiatria, v. 80, n. 2, p. 192-207, 2022-03-25. (15/25607-0, 13/07559-3, 19/25948-3, 19/00213-0, 18/00142-3)

GODOI, ALEXANDRE B.; DO CANTO, AMANDA M.; DONATTI, AMANDA; ROSA, DOUGLAS C.; BRUNO, DANIELLE C. F.; ALVIM, MARINA K.; YASUDA, CLARISSA L.; MARTINS, LUCAS G.; QUINTERO, MELISSA; TASIC, LJUBICA; et al. Circulating Metabolites as Biomarkers of Disease in Patients with Mesial Temporal Lobe Epilepsy. METABOLITES, v. 12, n. 5, p. 16-pg., 2022-05-01. (21/04451-3, 13/07559-3, 19/00213-0, 18/24069-3, 21/04441-8, 19/00048-0, 19/25948-3, 17/26167-0)