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Proteomic analysis of hippocampal tissues obtained from patients and animal model of mesial temporal lobe epilepsy: a comparative study

Grant number: 15/12960-4
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2015
Effective date (End): May 05, 2020
Field of knowledge:Health Sciences - Medicine
Principal Investigator:Iscia Teresinha Lopes Cendes
Grantee:Amanda Morato Do Canto
Home Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:13/07559-3 - BRAINN - The Brazilian Institute of Neuroscience and Neurotechnology, AP.CEPID
Associated scholarship(s):19/04335-3 - Identification of differentially phosphorylated proteins in the brain tissue resected from patients with mesial temporal lobe epilepsy with hippocampal sclerosis, BE.EP.DR

Abstract

Epilepsy is a neurological disorder caused by cerebral function alterations and it affects almost 2% of the world population. A common feature to all types of epilepsy is the occurrence of epileptics seizures due to abnormal neuronal discharges that occur in a briefly, synchronic and disorganized way, leading to clinical manifestation related to the nervous system affected area. The mesial temporal lobe epilepsy (TLE) is the most frequent type of epilepsy in adults and it is clinically characterized by a progressive development of seizures with focus on the temporal lobe and in the most cases it is associated with the hippocampal sclerosis. In this context, the most recent proteomic techniques give us powerful tools that allow us to find altered proteins in the organism as a response to intern and extern insults, changes in the development, etc. Therefore, our main goal is to analyze and compare changes at the protein level that occur in the hippocampal tissue from patients and animal models with MTLE and compare it with the normal controls. To refine the spatial resolution of the structures that will be studied inside the hippocampus we proposed to use the laser microdissection to isolate the sub-fields of the hippocampus and isolate cell populations of interest. We consider that our studies can provide important and original information about the molecular mechanisms involved with the hipocampal lesion that occur in the MTLE since it will be the first study to compare the findings in the patients tissue and animal model tissue with MTLE using proteomics approaches based on mass-spectrometry of high resolution. Beside that we propose to focus on specific techniques to identify membrane proteins and phosphorylated differentially expressed proteins.