Proteomic analysis of hippocampal tissues obtained from patients and animal model of Mesial Temporal Lobe Epilepsy: a comparative study

• Grant number: 15/12960-4
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): October 01, 2015
• Effective date (End): June 05, 2020
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Iscia Teresinha Lopes Cendes
• Grantee: Amanda Morato Do Canto
• Home Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 13/07559-3 - BRAINN - The Brazilian Institute of Neuroscience and Neurotechnology, AP.CEPID
• Associated scholarship(s): 19/04335-3 - Identification of differentially phosphorylated proteins in the brain tissue resected from patients with mesial temporal lobe epilepsy with hippocampal sclerosis, BE.EP.DR

Abstract

Epilepsy is a neurological disorder caused by cerebral function alterations and it affects almost 2% of the world population. A common feature to all types of epilepsy is the occurrence of epileptics seizures due to abnormal neuronal discharges that occur in a briefly, synchronic and disorganized way, leading to clinical manifestation related to the nervous system affected area. The mesial temporal lobe epilepsy (TLE) is the most frequent type of epilepsy in adults and it is clinically characterized by a progressive development of seizures with focus on the temporal lobe and in the most cases it is associated with the hippocampal sclerosis. In this context, the most recent proteomic techniques give us powerful tools that allow us to find altered proteins in the organism as a response to intern and extern insults, changes in the development, etc. Therefore, our main goal is to analyze and compare changes at the protein level that occur in the hippocampal tissue from patients and animal models with MTLE and compare it with the normal controls. To refine the spatial resolution of the structures that will be studied inside the hippocampus we proposed to use the laser microdissection to isolate the sub-fields of the hippocampus and isolate cell populations of interest. We consider that our studies can provide important and original information about the molecular mechanisms involved with the hipocampal lesion that occur in the MTLE since it will be the first study to compare the findings in the patients tissue and animal model tissue with MTLE using proteomics approaches based on mass-spectrometry of high resolution. Beside that we propose to focus on specific techniques to identify membrane proteins and phosphorylated differentially expressed proteins.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CANTO, AMANDA M.; MATOS, ALEXANDRE H. B.; GODOI, ALEXANDRE B.; VIEIRA, ANDRE S.; AOYAMA, BEATRIZ B.; ROCHA, CRISTIANE S.; HENNING, BARBARA; CARVALHO, BENILTON S.; PASCOAL, VINICIUS D. B.; VEIGA, DIOGO F. T.; GILIOLI, ROVILSON; CENDES, FERNANDO; LOPES-CENDES, ISCIA. Multi-omics analysis suggests enhanced epileptogenesis in theCornu Ammonis3 of the pilocarpine model of mesial temporal lobe epilepsy. HIPPOCAMPUS, v. 31, n. 2, p. 122-139, FEB 2021. Web of Science Citations: 1.

DO CANTO, AMANDA M.; DONATTI, AMANDA; GERALDIS, JAQUELINE C.; GODOI, ALEXANDRE B.; DA ROSA, DOUGLAS C.; LOPES-CENDES, ISCIA. Neuroproteomics in Epilepsy: What Do We Know so Far?. FRONTIERS IN MOLECULAR NEUROSCIENCE, v. 13, JAN 7 2021. Web of Science Citations: 0.

CANTO, AMANDA M.; VIEIRA, ANDRE S.; MATOS, ALEXANDRE H. B.; CARVALHO, BENILTON S.; HENNING, BARBARA; NORWOOD, BRAXTON A.; BAUER, SEBASTIAN; ROSENOW, FELIX; GILIOLI, ROVILSON; CENDES, FERNANDO; LOPES-CENDES, ISCIA. Laser microdissection-based microproteomics of the hippocampus of a rat epilepsy model reveals regional differences in protein abundances. SCIENTIFIC REPORTS, v. 10, n. 1 MAR 10 2020. Web of Science Citations: 0.