Alterations in transcriptome, metabolome and physiological responses associated with sugarcane drought memory

• Grant number: 19/27106-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: November 01, 2020
• End date: October 31, 2023
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Rafael Vasconcelos Ribeiro
• Grantee: Maria Dolores Pissolato
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

A recent study developed by our group demonstrated that sugarcane plants previously exposed to cycles of water deficit and rehydration or originated from material that had undergone water deficit had their photosynthetic performance and growth favored when exposed to drought again. The previous exposure to stressful events can trigger changes in the pattern of gene expression that regulate all plant development and allow a more efficient and faster response in cases of recurrent stress, and this phenomenon is treated in the literature as plant memory. The aim of this proposal is to identify the molecular, metabolic and physiological markers that would be associated with memory and drought in sugarcane and to evaluate their inheritance and persistence in vegetatively propagated plants. For this purpose, a large-scale analysis of the main groups of primary metabolites and transcriptome will be performed on samples of leaves, stem buds and sugarcane roots exposed to drought and rehydration cycles. The physiological and molecular responses of these plants will also be analyzed by associating them with the drought memory and their seedlings inheritance. Based on the obtained data, its pretend to suggest new management practices for pre-broken sugarcane seedlings in nursery condition, besides establishing a platform for transcriptomic analysis on that species, which will serve as a basis for the identification and understanding of molecular mechanisms that allow the inheritance of memory to environmental stresses in sugarcane. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALMEIDA, R. L.; SILVEIRA, N. M.; MIRANDA, M. T.; PACHECO, V. S.; CRUZ, L. P.; XAVIER, M. A.; MACHADO, E. C.; RIBEIRO, R. V.. Evidence of photosynthetic acclimation to self-shading in sugarcane canopies. Photosynthetica, v. 60, n. 4, p. 8-pg., 2022-10-20. (18/09834-5, 19/27106-0, 19/06161-2, 19/08047-2)

RIBEIRO, V, RAFAEL; VITTI, KARINE A.; MARCOS, FERNANDA C. C.; SOUZA, GUSTAVO M.; PISSOLATO, MARIA D.; ALMEIDA, LUIZ FERNANDO R.; MACHADO, EDUARDO C.. Proposal of an index of stability for evaluating plant drought memory: A case study in sugarcane. Journal of Plant Physiology, v. 260, MAY 2021. (19/27106-0, 18/21194-1, 19/06161-2, 19/08047-2)

MARIA DOLORES PISSOLATO; LARISSA PRADO DA CRUZ; NEIDIQUELE MARIA SILVEIRA; EDUARDO CARUSO MACHADO; RAFAEL VASCONCELOS RIBEIRO. Sugarcane regrowth is dependent on root system size: an approach using young plants grown in nutrient solution. Bragantia, v. 80, 2021-08-13. (19/27106-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PISSOLATO, Maria Dolores. Respostas metabólicas e fisiológicas associadas a memória somática e intergeracional à seca em cana-de-açúcar. 2024. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.