Molecular epidemiology of G8 rotavirus strains isolated in Brazil associated with P[4], P[6] or P[8] genotypes

• Grant number: 20/11182-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2021
• End date: December 31, 2021
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Adriana Luchs
• Grantee: Roberta Salzone Medeiros
• Host Institution: Instituto Adolfo Lutz (IAL). Coordenadoria de Controle de Doenças (CCD). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil

Abstract

Group A rotaviruses (RVA) are the main cause of acute gastroenteritis in humans. RVAs are species-specific; however interspecies transmission and rearrangement between human and animal RVAs contribute to their genetic diversity. The G8 genotype is widely distributed in animal species, being reported in pigs, horses, but mainly in cattle. The detection of G8 strains infecting humans is rare, nevertheless reported worldwide, including in Brazil. The origin of the G8 strains in the human population has been investigated in recent years and a potential bovine origin is suggested. G8 strains exhibit an ability to repeatedly combine with VP4 strains ecologically adapted to the human gut (ie, P[4], P[6], and P[8]), which could facilitate their dispersion in the human population. Human G8P[8] strains may possess both Wa-like and DS-1-like genomic constellation, and recently, G8P[8] DS-1-like strains with bovine G8 origin have emerged in Asia. The emergence of strains exhibiting unusual and/or interspecific rearrangements can lead to future implications in vaccination programs. The RVA monitoring conducted by the Adolfo Lutz Institute in the last 10 years (2009-2019) permitted the identification of 15 unusual RVA G8 strains associated with P[4] (5 samples), P[6] (7 samples), and P[8] (3 samples). The aims of the present study is to conduct the molecular characterization and phylogenetic analysis of the 11 segments (genomic constellation) of RVA G8P[4]/P[6]/P[8] strains detected between 2009 and 2017, targeting to reveal the true origin of these strains, as well as understanding their ability to evade vaccine immunity. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIANA PIRES DE SOUZA, YASMIN FRANCA; DE SOUZA, ELLEN VIANA; DE AZEVEDO, LAIS SAMPAIO; MEDEIROS, ROBERTA SALZONE; SAMPAIO TAVARES TIMENETSKY, MARIA DO CARMO; LUCHS, ADRIANA. Enteric adenovirus epidemiology from historical fecal samples in Brazil (1998-2005): Pre-rotavirus vaccine era. INFECTION GENETICS AND EVOLUTION, v. 94, OCT 2021. (20/11182-6, 20/02469-0)

DE AZEVEDO, LAIS SAMPAIO; FRANCA, YASMIN; VIANA, ELLEN; MEDEIROS, ROBERTA SALZONE; MORILLO, SIMONE GUADAGNUCCI; GUIDUCCI, RAQUEL; RIBEIRO, CIBELE DANIEL; VIEIRA, HELOISA ROSA; BARRIO-NUEVO, KAROLINA MORALES; CUNHA, MARIANA SEQUETIN; et al. Lack of molecular evidence of fecal-borne viruses in capybaras from Sao Paulo state, Brazil, 2018-2020: a minor public health issue. Brazilian Journal of Microbiology, v. N/A, p. 9-pg., 2022-11-07. (20/14786-0, 20/02469-0, 15/12944-9, 20/11182-6, 19/21706-5)

LUCHS, ADRIANA; TARDY, KAELAN; TAHMASEBI, ROOZBEH; MORILLO, SIMONE GUADAGNUCCI; MILAGRES, FLAVIO AUGUSTO DE PADUA; MORAIS, VANESSA DOS SANTOS; BRUSTULIN, RAFAEL; TELES, MARIA DA APARECIDA RODRIGUES; DE AZEVEDO, LAIS SAMPAIO; DE SOUZA, ELLEN VIANA; et al. Human astrovirus types 1, 4 and 5 circulating among children with acute gastroenteritis in a rural Brazilian state, 2010-2016. ARCHIVES OF VIROLOGY, v. 166, n. 11, AUG 2021. (19/21706-5, 15/12944-9, 17/00021-9, 20/11182-6, 20/02469-0, 16/01735-2)

ADRIANA LUCHS; LAIS SAMPAIO DE AZEVEDO; ELLEN VIANA DE SOUZA; ROBERTA SALZONE MEDEIROS; YASMIN FRANÇA VIANA PIRES DE SOUZA; DALANE LOUDAL FLORENTINO TEIXEIRA; THIAGO FRANCO DE OLIVEIRA CARNEIRO; GABRIELA MARIA FERNANDES DE ALENCAR; FERNANDA LÚCIA DE SOUSA LEITE MORAIS; DIANA DE FÁTIMA ALVES PINTO; et al. Coxsackievirus A6 strains causing an outbreak of hand-foot-and-mouth disease in Northeastern Brazil in 2018. Revista do Instituto de Medicina Tropical de São Paulo, v. 64, 2022-02-21. (20/02469-0, 17/00021-9, 19/21706-5, 20/14786-0, 20/11182-6)

CUNHA, LIA; LUCHS, ADRIANA; AZEVEDO, LAIS S. S.; SILVA, VANESSA C. M.; LEMOS, MARCILIO F. F.; COSTA, ANTONIO C. C.; COMPRI, ADRIANA P. P.; FRANCA, YASMIN; VIANA, ELLEN; MALTA, FERNANDA; et al. Detection of Hepatitis E Virus Genotype 3 in Feces of Capybaras (Hydrochoeris hydrochaeris) in Brazil. Viruses-Basel, v. 15, n. 2, p. 13-pg., 2023-02-01. (20/02469-0, 15/12944-9, 20/14786-0, 20/11182-6)

AZEVEDO, LAIS SAMPAIO; COSTA, FERNANDA FARIA; GHANI, MONIQUE BEERENS ABDUL; VIANA, ELLEN; FRANCA, YASMIN; MEDEIROS, ROBERTA SALZONE; GUIDUCCI, RAQUEL; MORILLO, SIMONE GUADAGNUCCI; PRIMO, DIELI; LOPES, RICARDO DUARTE; et al. Full genotype characterization of Brazilian canine G3P[3] strains during a 10-year survey (2012-2021) of rotavirus infection in domestic dogs and cats. ARCHIVES OF VIROLOGY, v. 168, n. 7, p. 15-pg., 2023-07-01. (20/02469-0, 15/12944-9, 20/14786-0, 20/11182-6)

MEDEIROS, ROBERTA SALZONE; FRANCA, YASMIN; VIANA, ELLEN; DE AZEVEDO, LAIS SAMPAIO; GUIDUCCI, RAQUEL; DE LIMA NETO, DANIEL FERREIRA; DA COSTA, ANTONIO CHARLYS; LUCHS, ADRIANA. Genomic Constellation of Human Rotavirus G8 Strains in Brazil over a 13-Year Period: Detection of the Novel Bovine-like G8P[8] Strains with the DS-1-like Backbone. Viruses-Basel, v. 15, n. 3, p. 34-pg., 2023-03-01. (20/02469-0, 15/12944-9, 20/14786-0, 20/11182-6)