Production of bacteriocin-producing probiotic bacteria

• Grant number: 20/13271-6
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Start date: November 01, 2020
• End date: May 31, 2024
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Ricardo Pinheiro de Souza Oliveira
• Grantee: Carlos Miguel Nóbrega Mendonça
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems, AP.TEM
• Associated scholarship(s): 21/13413-8 - Evaluating the production and efficacy of bacteriocins by potential probiotic bacteria isolated from chicken gut using dynamic in vitro gastrointestinal tract models, BE.EP.DD

Abstract

The use of probiotic microorganisms in the prevention and treatment of bacterial infections in animals intended for human consumption has been considered an efficient alternative to the use of antibiotics. In addition, recent studies have shown that certain substances produced by these microorganisms, such as bacteriocins, vitamins, fatty acids, exopolysaccharides, enzymes, among others, improve the immunity and development of their hosts. The most commonly used probiotic microorganisms in the food and pharmaceutical industries are those belonging to the Lactic Acid Bacteria group (LAB), since they are considered safe by regulatory agencies in this area. However, it is known that the beneficial effects generated by probiotics are specific to each host and, frequently, the compounds of interest are synthesized in greater quantities by a given bacterial strain. The main objective of the present study is the isolation and identification of probiotic LAB present in the intestinal microbiota of poultry, swine and fish species. Strains with high capacity to produce bacteriocins will be selected. From this selection, compatibility tests will be carried out among the strains and, later, a mix of probiotics will be prepared. The strains that compose it will be individually micro-encapsulated and administered daily in the animals diet, through feed and water, in order to ascertain the probiotic effectiveness of the mixture. Synthetic bacteriocins will also be micro-encapsulated and administered to understand their individual effect on animals health. The results obtained with microcapsules will be compared with those obtained in the same biosystems in free form. In addition, we will carry out immunological studies, electron microscopy tests and analysis of the intestinal microbiota diversity of animals. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VERISSIMO, NATHALIA VIEIRA; MUSSAGY, CASSAMO USSEMANE; OSHIRO, ARIANE ALVES; NOBREGA MENDONCA, CARLOS MIGUEL; SANTOS-EBINUMA, VALERIA DE CARVALHO; PESSOA JUNIOR, ADALBERTO; DE SOUZA OLIVEIRA, RICARDO PINHEIRO; BRANDAO PEREIRA, JORGE FERNANDO. From green to blue economy: Marine biorefineries for a sustainable ocean-based economy. GREEN CHEMISTRY, v. 23, n. 23, NOV 2021. (18/06908-8, 20/13271-6, 20/14144-8, 19/15493-9, 18/25511-1, 20/08655-0)

DE OLIVEIRA, RODRIGO CARDOSO; NOBREGA MENDONCA, CARLOS MIGUEL; VERISSIMO, NATHALIA VIEIRA; YOKOMIZO DE ALMEIDA, SONIA REGINA; CORREA, BENEDITO; WATANABE, II-SEI; DE SOUZA DE AZEVEDO, PAMELA OLIVEIRA; DE SOUZA OLIVEIRA, RICARDO PINHEIRO. Evaluating the potential of Pediococcus pentosaceus as a biocontrol agent against tenuazonic acid-producing Alternaria alternata on livestock feeds. Journal of Applied Microbiology, v. 133, n. 5, p. 10-pg., 2022-08-10. (19/16551-2, 20/13271-6, 18/25511-1, 20/14144-8, 20/10676-5, 19/02456-8)

MENDONCA, CARLOS M. N.; OLIVEIRA, RODRIGO C.; PIZAURO, LUCAS J. L.; PEREIRA, WELLISON A.; ABBOUD, KAHLILE; ALMEIDA, SONIA; WATANABE, II-SEI; VARANI, ALESSANDRO M.; DOMINGUEZ, JOSE M.; CORREA, BENEDITO; et al. Tracking new insights into antifungal and anti-mycotoxigenic properties of a biofilm forming Pediococcus pentosaceus strain isolated from grain silage. International Journal of Food Microbiology, v. 405, p. 10-pg., 2023-11-16. (18/25511-1, 20/13271-6, 21/15138-4)

SANCA, FERNANDO M. M.; BLANCO, IAGO R.; DIAS, MERIELLEN; MORENO, ANDREA M.; MARTINS, SIMONE M. M. K.; STEPHANO, MARCO A.; MENDES, MARIA A.; MENDONCA, CARLOS M. N.; PEREIRA, WELLISON A.; AZEVEDO, PAMELA O. S.; et al. Antimicrobial Activity of Peptides Produced by Lactococcus lactis subsp. lactis on Swine Pathogens. ANIMALS, v. 13, n. 15, p. 12-pg., 2023-08-01. (18/25511-1, 22/07304-4, 22/07543-9, 20/13271-6, 22/07556-3, 22/04841-9)

PEREIRA, WELLISON AMORIM; PIAZENTIN, ANNA CAROLINA M.; DE OLIVEIRA, RODRIGO CARDOSO; MENDONCA, CARLOS MIGUEL N.; TABATA, YARA AIKO; MENDES, MARIA ANITA; FOCK, RICARDO AMBROSIO; MAKIYAMA, EDSON NAOTO; CORREA, BENEDITO; VALLEJO, MARISOL; et al. Bacteriocinogenic probiotic bacteria isolated from an aquatic environment inhibit the growth of food and fish pathogens. SCIENTIFIC REPORTS, v. 12, n. 1, p. 14-pg., 2022-04-01. (20/13271-6, 18/25511-1, 20/10676-5, 21/01570-1)

LARA, VIRGINIA M.; MENDONCA, CARLOS M. N.; SILVA, FRANCISCO V. S.; MARGUET, EMILIO R.; VALLEJO, MARISOL; CONVERTI, ATTILIO; VARANI, ALESSANDRO M.; GLIEMMO, MARIA F.; CAMPOS, CARMEN A.; OLIVEIRA, RICARDO P. S.. Characterization of Lactiplantibacillus plantarum Tw226 strain and its use for the production of a new membrane-bound biosurfactant. JOURNAL OF MOLECULAR LIQUIDS, v. 363, p. 10-pg., 2022-07-26. (20/13271-6, 18/25511-1)

MENDONC, CARLOS M. N.; VERISSIMO, NATHALIA V.; PEREIRA, WELLISON A. .; CUNHA, PAULA M.; VITOLO, MICHELE; CONVERTI, ATTILIO; KURNIA, KIKI ADI; SEGATO, FERNANDO; AZEVEDO, PAMELA O. S. DE; FREIRE, MARA G.; et al. Use of Tunable Copolymers in Aqueous Biphasic Systems for Extractive Bioconversion Aimed at Continuous Fructooligosaccharide Production. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. N/A, p. 14-pg., 2023-01-11. (20/13271-6, 18/25511-1)

MENDONCA, CARLOS M. N.; OLIVEIRA, RODRIGO C.; FREIRE, ROMINNE K. B.; PIAZENTIN, ANNA C. M.; PEREIRA, WELLISON A.; GUDINA, EDUARDO J.; EVTUGUIN, V, DMITRY; CONVERTI, ATTILIO; SANTOS, JOAO H. P. M.; NUNES, CLAUDIA; et al. Characterization of levan produced by a Paenibacillus sp. isolated from Brazilian crude oil. International Journal of Biological Macromolecules, v. 186, p. 788-799, SEP 1 2021. (20/13271-6, 18/25511-1)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MENDONÇA, Carlos Miguel Nóbrega. The prevention as an approach to promoting sanitary safety and sustainability in poultry farming: emphasis on precision nutrition and animal gastrointestinal health. 2024. Doctoral Thesis - Universidade de São Paulo (USP). Conjunto das Químicas (IQ e FCF) (CQ/DBDCQ) São Paulo.