Advanced search
Start date
Betweenand

Study of the expression of oncogenes in co-culture of BEAS-2B cells and macrophage type 1 or type 2 exposed to anthracosis and atmospheric particulate matter (PM2.5)

Grant number: 19/03586-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2020
Effective date (End): September 30, 2022
Field of knowledge:Health Sciences - Collective Health - Public Health
Principal researcher:Mariangela Macchione
Grantee:Daniela Perroni Frias
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Anthracosis is the black discoloration of the lungs and tracheobronchial tree caused by deposition of inhaled carbon particles. There is a hypothesis that this condition is the result of epithelial damage in different areas, leading to aberrant scarring and fibrosis in the lungs and bronchial wall. Recent evidence indicates that particulate air pollution plays an important role in the establishment of anthracosis and possibly in the development of cancer in the respiratory system, due to the carcinogenic substances from the air pollution that remain deposited. The aim of this work is to study the expression of oncogenes in co-culture of normal human bronchial cells (BEAS-2B) and type 1 and type 2 macrophages exposed to environmental pollution particulate matter and anthracosis. For this study, the environmental particulate material will be collected in polycarbonate filters near from a region of large vehicular flow and the anthracosis material will be collected from the corpse lung of the Sao Paulo University Capital Death Certificates Service (SVO). After exposure of the co-culture to different concentrations of each particulate, viability tests (MTT and LDH) will be performed and the expressions of 84 oncogenes will be evaluated by microarray kit. Of these genes, those with more altered expression will be tested for differentiated methylation of CpG regions by RT-PCR and Western Blotting for quantification of proteins.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)