Anthracosis is the black discoloration of the lungs and tracheobronchial tree caused by deposition of inhaled carbon particles. There is a hypothesis that this condition is the result of epithelial damage in different areas, leading to aberrant scarring and fibrosis in the lungs and bronchial wall. Recent evidence indicates that particulate air pollution plays an important role in the establishment of anthracosis and possibly in the development of cancer in the respiratory system, due to the carcinogenic substances from the air pollution that remain deposited. The aim of this work is to study the expression of oncogenes in co-culture of normal human bronchial cells (BEAS-2B) and type 1 and type 2 macrophages exposed to environmental pollution particulate matter and anthracosis. For this study, the environmental particulate material will be collected in polycarbonate filters near from a region of large vehicular flow and the anthracosis material will be collected from the corpse lung of the Sao Paulo University Capital Death Certificates Service (SVO). After exposure of the co-culture to different concentrations of each particulate, viability tests (MTT and LDH) will be performed and the expressions of 84 oncogenes will be evaluated by microarray kit. Of these genes, those with more altered expression will be tested for differentiated methylation of CpG regions by RT-PCR and Western Blotting for quantification of proteins.
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