Evaluation of the intestinal gene expression of SCARB1 and BCO1 in obese women submitted to Roux-en-Y gastric bypass and its association with vitamin A deficiency

• Grant number: 20/06888-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2020
• End date: November 30, 2021
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Priscila Sala Kobal
• Grantee: Priscilla Maciel Sampaio Campos
• Host Institution: Campus Pompéia. Centro Universitário São Camilo (CUSP). União Social Camiliana (USC). São Paulo , SP, Brazil

Abstract

Bariatric surgery is a surgical procedure frequently used to treat obesity and its comorbidities. Roux-en-Y gastric bypass (RYGB) is the gold standard bariatric surgery technique, which involves gastric reduction and intestinal bypass. Nutritional deficiencies are among the most common complications of bariatric surgery, including vitamin A deficiency, which may reach 11% after 1 year of RYGB, even with vitamin supplementation. Experimentally, the anatomical changes induced by the RYGB technique may influence the absorption of this micronutrient, since vitamin A is mainly absorbed in the duodenum and proximal jejunum, a segment excluded from the nutrient flux after RYGB. Two key genes are involved in vitamin A metabolism: the SCARB1 gene responsible for the passage of ²-carotene into the duodenum and the protein encoded by the BCO1 gene is the key enzyme that converts ²-carotene in vitamin A. In the present study, genes related to vitamin A metabolism will be analyzed in intestinal biopsies of patients submitted to RYGB, as well as the amount of supplementation received by these patients and plasma vitamin A concentrations. The study of the expression of genes involved in the metabolism of vitamin A in the intestinal tissue of patients after RYGB may lead us to better understand the potential mechanisms that contribute to the deficiency of this micronutrient.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SAMPAIO, PRISCILLA; WAITZBERG, DAN LINETZKY; MACHADO, NATASHA MENDONCA; MATOS DE MIRANDA TORRINHAS, RAQUEL SUSANA; FONSECA, DANIELLE C.; FERREIRA, BEATRIZ A. M.; MARQUES, MARIANE; BARCELOS, SAMIRA; ISHIDA, ROBSON KIYOSHI; MOTA SIQUEIRA GUARDA, ISMAEL FRANCISCO; et al. Gastrointestinal genetic reprogramming of vitamin A metabolic pathways in response of Roux-en-Y gastric bypass. INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH, v. N/A, p. 10-pg., 2022-09-27. (20/06888-7, 11/09612-3)