Proteomic analysis of the systemic effects of Bothrops jararaca venom in a murine model

• Grant number: 20/12317-2
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: December 01, 2020
• End date: November 30, 2025
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Solange Maria de Toledo Serrano
• Grantee: Alison Felipe Alencar Chaves
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling, AP.CEPID
• Associated scholarship(s): 21/14329-0 - Multi-omics approach to address the systemic effects of Bothrops jararaca venom in a murine model, BE.EP.PD

Abstract

Snakebite envenomation is recognized in category A of the Neglected Tropical Diseases by the World Health Organization (WHO) due to the worldwide burden of snakebite death and disability. Although the local effects of bites are well documented, knowledge on the systemic effects of snake venoms is limited. Envenomation by Viperidae snakes causes hypotension, ultimately leading to shock, coagulopathy, and kidney failure, but the mechanisms behind these effects remain broadly unknown. This mismatch between clinical and mechanistic knowledge makes it hard to find better interventions for severe envenoming with systemic effects. By using an integrative approach including quantitative proteomics, N-terminomics, and phosphoproteomics, in animals which will receive or not bothropic antivenom after Bothrops jararaca venom injection, we will analyze altered pathways in kidneys, liver, and lungs, with special focus on the activity of venom proteolytic enzymes.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ASEGA, AMANDA F.; BARROS, BIANCA C. S. C.; CHAVES, ALISON F. A.; OLIVEIRA, ANA K.; BERTHOLIM, LUCIANA; KITANO, EDUARDO S.; SERRANO, SOLANGE M. T.. Mouse skin peptidomic analysis of the hemorrhage induced by a snake venom metalloprotease. Amino Acids, v. 55, n. 9, p. 17-pg., 2023-06-30. (21/10570-5, 13/07467-1, 20/12317-2, 10/17328-0, 11/11308-0, 11/16623-1, 10/00206-0)

BERTHOLIM, LUCIANA; CHAVES, ALISON F. A.; OLIVEIRA, ANA K.; MENEZES, MILENE C.; ASEGA, AMANDA F.; TASHIMA, ALEXANDRE K.; ZELANIS, ANDRE; SERRANO, SOLANGE M. T.. Systemic Effects of Hemorrhagic Snake Venom Metalloproteinases: Untargeted Peptidomics to Explore the Pathodegradome of Plasma Proteins. TOXINS, v. 13, n. 11, NOV 2021. (11/08514-8, 20/12317-2, 11/16623-1, 13/07467-1)