Effects of Aerobic and Anaerobic exercises on RedOx state modulation in skeletal muscle using animal model of Metabolic Syndrome

• Grant number: 20/06108-1
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: February 01, 2021
• End date: December 31, 2022
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Camila Renata Corrêa
• Grantee: Bruno Henrique de Paula
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Metabolic syndrome (MetS) is characterized by a constellation of cardiovascular risk factors, such as systemic arterial hypertension, insulin resistance, central obesity, hyperlipidemia and low levels of HDL-cholesterol The dramatic increase in individuals with MetS has been recognized as public health problem in the world today. Physical exercise has been used as a non-pharmacological therapy for the treatment and prevention of MS due to its antioxidant capacity. Objective: To compare the effects of Aerobic and Anaerobic exercises on NRF-2 protein expression by modulating the RedOx state in skeletal muscle in an animal model of metabolic syndrome. Methods: Wistar rats (n = 60) with approximately 180 grams will be used, which will be randomly distributed in two groups (n = 30) to receive control ration + water (control diet [DC]) or ration rich in simple carbohydrates and fat + drinking water plus 25% sucrose (High Sugar-Fat Diet [HSF]), making up the control groups (C) and HSF. The animals will receive the diets throughout the experimental period, and in the 20th week they will be submitted to the analysis of the components of the metabolic syndrome. The syndrome was confirmed by the assessment of caudal systolic blood pressure, blood glucose, triacylglycerol and insulin resistance, which will be calculated using the HOMA-IR index. The animals will be redistributed into six experimental groups (n = 10), being: control (C), control + aerobic exercise (C + EFA), control + resistance exercise (C + EFR), metabolicsyndrome (SM), SM + aerobic physical exercise (SM + EFA) and SM + resistance physical exercise (SM + EFR). The trial period will have a total duration of 30 weeks.