Development of wearable sensors based on nanomaterials to detect fungal diseases in plants

• Grant number: 20/10048-4
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2021
• End date: February 28, 2026
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal Investigator: Daniel Souza Corrêa
• Grantee: Tamires dos Santos Pereira
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
• Associated research grant: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM
• Associated scholarship(s): 23/16193-4 - Thin film transistors (TFTs) using nanomaterials and low-cost substrates for monitoring analytes relevant to the agroindustry, BE.EP.DR

Abstract

Fungal diseases annually cause losses in agricultural production and great economic loss. In Brazil, cultures with commercial importance are also affected by pathologies caused by these microorganisms. The diagnosis at the initial stage of infection in plants can mean greater efficiency in controlling the disease, with a consequent increase in productivity and economic gains. In this sense, analytical techniques, such as the use of sensors that allow rapid, continuous, easy to handle, low-cost and in situ diagnosis are of great relevance. This project aims at developing "wearable sensors" for application in plants aiming at the detection of diseases. The sensors will be prepared using strategies such as electrospinning, using polymers such as polyamide and poly (lactic acid), modified with conductive materials suchas conjugated polymers, metallic nanoparticles and carbonaceous nanomaterials, deposited on electrodes that will be coupled to a wearable platform manufactured by a 3D printer, suitable for fixation on plants. The nanofibers and active nanomaterials employed will be characterized in relation to their morphology, structure, mechanical, optical and electrical properties. For this purpose, techniques such as Scanning Electron Microscopy (SEM), Atomic Force Microscopy (AFM), Infrared Spectroscopy (FITR), Thermal Analysis (DSCand TGA), X-Ray Diffraction (XRD), Tensile tests, UV-Vis, fluorescence and impedance spectroscopy will be employed. The analytes of interest to be detected include defense metabolites released by plants in the early stages of the disease. Quantification will be performed by measurements of color change and/or electrical resistance, before and after contact with the analytes. It is expected that this project can contribute with strategies to advance the early diagnosis of plant diseases. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MERCANTE, LUIZA A.; PAVINATTO, ADRIANA; PEREIRA, TAMIRES S.; MIGLIORINI, FERNANDA L.; DOS SANTOS, DANILO M.; CORREA, DANIEL S.. Nanofibers interfaces for biosensing: Design and applications. SENSORS AND ACTUATORS REPORTS, v. 3, NOV 2021. (17/20973-4, 18/22214-6, 17/21791-7, 20/10048-4)

TEODORO, KELCILENE B. R.; PEREIRA, TAMIRES S.; ALVES, ANA LAURA M. M.; DOS SANTOS, FRANCISCO V.; DOS SANTOS, FABRICIO A.; CORREA, DANIEL S.. 3D-printed microneedles for sensing applications: Emerging topics and future trends. ADVANCED SENSOR AND ENERGY MATERIALS, v. 4, n. 2, p. 22-pg., 2025-05-12. (23/01567-6, 22/09686-1, 23/13428-0, 22/05316-5, 20/10048-4)

PEREIRA, TAMIRES DOS SANTOS; DOS SANTOS, DANILO MARTINS; ANDRE, RAFAELA DA SILVEIRA; CORREA, DANIEL SOUZA. Zein/MnO₂ Nanosheet Composites Integrated with a Smartphone for Colorimetric Sensors for On-Site Detection of Adulterants in Milk. ACS APPLIED NANO MATERIALS, v. 7, n. 11, p. 11-pg., 2024-06-04. (18/22214-6, 20/10048-4, 17/20973-4, 16/23793-4)