Conductive composite microneedles manufactured by 3D printing and applications in wearable biosensors

• Grant number: 23/13428-0
• Support Opportunities: Scholarships in Brazil - Support Program for Fixating Young Doctors
• Start date: November 01, 2023
• End date: October 31, 2025
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Agreement: CNPq
• Principal Investigator: Daniel Souza Corrêa
• Grantee: Kelcilene Bruna Teodoro Costa
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
• Associated research grant: 23/01567-6 - Conductive composite microneedles manufactured by 3D printing and applications in wearable biosensors, AP.R

Abstract

In this project, we propose to develop conductive polymeric microneedles (MN) to be applied as electrodes for (bio)sensors for minimally invasive monitoring of different analytes. Despite the growing popularity of these platforms, efforts have been focused on coating them with conducting polymers or using metallic microneedles. These materials lack biological stability and require laborious and costly fabrication methods, creating translational barriers. In the present proposal, 3D resin printing, which provides the user with versatility, simplicity, precision, and the ability to print on demand, will be used to manufacture microneedle devices from conductive photocurable inks produced from zein methacrylate and quantum dots of carbon synthesized from biomass, such as lignin. By utilizing statistical tools such as factorial design and response surface methodology, we will optimize the ink formulation in terms of composition, ensuring high printability and microneedle arrays with mechanical and electrical properties suitable for sensor applications. We will use the best architecture determined by optimizing microneedle manufacturing conditions to develop an electrochemical sensor to detect levododa (L-dopa), a drug widely used to treat Parkinson's disease, which will serve as a model analyte for validating the effectiveness of the proposed platforms. We hope to advance the development of biocompatible, low-cost conductive microneedles for the construction of wearable (bio)sensors, which are potentially useful not only for monitoring human health biomarkers, but also for the detection of analytes associated with animal health, plant health, as well as food quality control.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVARENGA, AUGUSTO D.; LIMA, BRUNO DE A.; TEODORO, KELCILENE B. R.; DA COSTA, VITTOR P. V.; AGUIAR, MONICA L.; CORREA, DANIEL S.. Effect of static electricity and thickness of solution blow spun polyamide 6/ AgNP nanofibrous membrane on nanoparticle filtration and microbiological activity. Applied Surface Science, v. 652, p. 13-pg., 2024-01-09. (18/22214-6, 23/13428-0, 18/26069-0)

CHANG, YURU; FERREIRA, MARCOS DAVID; CORREA, DANIEL SOUZA; TEODORO, KELCILENE BRUNA RICARDO; PROCOPIO, FERNANDA RAMALHO; BREXO, RAMON PERES; SARKHOSH, ALI; BRECHT, JEFFREY K.. Advances in postharvest nanotechnology: Enhancing fresh produce shelf life and quality to reduce losses and waste. Postharvest Biology and Technology, v. 222, p. 25-pg., 2025-01-20. (23/17653-9, 22/10686-6, 23/14371-2, 18/22214-6, 23/01567-6, 23/13428-0)

ROSSI, PATRICIA F.; DOS SANTOS, DANILO M.; MARANGON, CRISIANE A.; TEODORO, KELCILENE B. R.; COSTA, CAMILA S.; INADA, NATALIA M.; CORREA, DANIEL S.; OREFICE, RODRIGO L.. Biobased composite fibrous membrane using PLA and lignin carbon dots fabricated via solution blow spinning for wound dressing application. MATERIALS TODAY COMMUNICATIONS, v. 42, p. 12-pg., 2025-01-01. (18/22214-6, 23/13428-0, 13/07276-1)

MERCANTE, LUIZA A.; TEODORO, KELCILENE B. R.; DOS SANTOS, DANILO M.; DOS SANTOS, FRANCISCO V.; BALLESTEROS, CAMILO A. S.; JU, TIAN; WILLIAMS, GARETH R.; CORREA, DANIEL S.. Recent Progress in Stimuli-Responsive Antimicrobial Electrospun Nanofibers. POLYMERS, v. 15, n. 21, p. 26-pg., 2023-11-01. (17/10582-8, 23/13428-0, 17/20973-4, 23/01567-6, 21/11738-7, 22/05316-5, 18/22214-6)

SILVA, JONATAS DE OLIVEIRA S.; CAPISTRANO, ANA VITORIA L.; TEODORO, KELCILENE B. R.; CORREA, DANIEL S.; SANTANA, RODOLFO M. M.; MERCANTE, LUIZA A.. Microfibrillated cellulose-based colorimetric sensor strips for detecting total iron in water. International Journal of Biological Macromolecules, v. 299, p. 9-pg., 2025-01-21. (23/01567-6, 18/22214-6, 23/13428-0)

TEODORO, KELCILENE BRUNA RICARDO; ALVARENGA, AUGUSTO D.; MARQUES CHAGAS, PAULO AUGUSTO; LOPES, RAPHAEL GUIMARAES; ALVES, FERNANDA; STRINGASCI, MIRIAN D.; HARB BUZZA, HILDE; INADA, NATALIA M.; CORREA, DANIEL SOUZA. Synergistic Tetracycline-Curcumin Association in Biocompatible PCL Nanostructured Wound Dressings. ACS APPLIED POLYMER MATERIALS, v. 6, n. 9, p. 13-pg., 2024-04-17. (13/07276-1, 23/13428-0, 18/22214-6)