Virological characterization of Brazilian SARS-CoV-2 isolates and their neutralization capabilities by serum from previously infected and/or vaccinated individuals

• Grant number: 21/05661-1
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2021
• End date: November 30, 2025
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Luiz Mário Ramos Janini
• Grantee: Robert Andreata Santos
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant: 20/08943-5 - Investigation of the hosts' induced elements in response to the immunisation with ChAdOx1 nCOV-19 vaccine in a Phase III Clinical Trial, AP.TEM
• Associated scholarship(s): 23/02345-7 - Evaluation of SARS-CoV-2 escape mutants generated after incubation with antibodies raised by different immunization protocols, BE.EP.PD

Abstract

The postdoctoral fellow to participate in this thematic project must have intellectual participation and work capacity to develop the central objectives of the project "Virological characterization of Brazilian isolates of SARS-CoV-2 and their capacity for neutralization by serum of previously infected individuals and /or vaccinated". In this project, it is of fundamental importance to monitor the different variants of SARS-CoV-2 isolated in Brazil, comparing them in different cell lines. Based on these accurate and real-time analyses, we will look at the dynamics of different isolates against sera from previously infected and/or vaccinated individuals. The associated data will be analyzed regarding the capacity of neutralizing the variants and a possible vaccine escape, prospecting elementary measures for the improvement of future therapeutic mechanisms. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ANDREATA-SANTOS, ROBERT; JANINI, LUIZ M. R.; DURAES-CARVALHO, RICARDO. From Alpha to Omicron SARS-CoV-2 variants: What their evolutionary signatures can tell us?. Journal of Medical Virology, JAN 2022. (21/03684-4, 19/01255-9, 21/05661-1, 20/08943-5)

SOUZA, MILENA SILVA; FARIAS, JESSICA PIRES; ANDREATA-SANTOS, ROBERT; SILVA, MARIANNE PEREIRA; BRITO, RUTH DALETY DA SILVA; DUARTE BARBOSA DA SILVA, MARCIA; PETER, CRISTINA MENDES; CIRILO, MARCUS VINICIUS DE FRANCA; LUIZ, WILSON BARROS; BIRBRAIR, ALEXANDER; et al. Neutralizing antibody response after immunization with a COVID-19 bivalent vaccine: Insights to the future. Journal of Medical Virology, v. 96, n. 2, p. 9-pg., 2024-02-01. (20/06409-1, 23/01925-0, 20/08943-5, 22/11981-1, 21/05661-1, 20/05204-7)

ANDREATA-SANTOS, ROBERT; MACHADO, RAFAEL RAHAL GUARAGNA; DOS SANTOS ALVES, RUBENS PRINCE; SALES, NATIELY SILVA; SOARES, CAMILA PEREIRA; RODRIGUES, KARINE BITENCOURT; SILVA, MARIANGELA OLIVEIRA; FAVARO, MARIANNA TEIXEIRA DE PINHO; RODRIGUES-JESUS, MONICA JOSIANE; YAMAMOTO, MARCIO MASSAO; et al. Validation of Serological Methods for COVID-19 and Retrospective Screening of Health Employees and Visitors to the Sao Paulo University Hospital, Brazil. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 12, p. 8-pg., 2022-06-02. (20/08943-5, 16/20045-7, 21/05661-1, 14/50890-5, 16/23560-0, 15/02352-7, 20/06409-1, 17/24769-2, 16/14344-1, 18/23680-0, 20/10700-3, 18/07629-5, 18/07142-9)

DA COSTA, ANDERSON LUIS; PRIETO-OLIVEIRA, PAULA; DUARTE-BARBOSA, MARCIA; ANDREATA-SANTOS, ROBERT; PETER, CRISTINA M.; DE BRITO, THAMIRES PROLO; ANTONELI, FERNANDO; DURAES-CARVALHO, RICARDO; BRIONES, MARCELO R. S.; MARICATO, JULIANA T.; et al. The Relationship between HERV, Interleukin, and Transcription Factor Expression in ZIKV Infected versus Uninfected Trophoblastic Cells. CELLS, v. 13, n. 17, p. 15-pg., 2024-09-01. (23/07287-5, 23/10230-5, 21/03684-4, 20/08943-5, 21/05661-1, 19/01255-9)

RODRIGUES-JESUS, MONICA JOSIANE; FAVARO, MARIANNA TEIXEIRA DE PINHO; VENCESLAU-CARVALHO, ALEXIA ADRIANNE; DE CASTRO-AMARANTE, MARIA FERNANDA; ALMEIDA, BIANCA DA SILVA; SILVA, MARIANGELA DE OLIVEIRA; ANDREATA-SANTOS, ROBERT; BARBOSA, CECILIA GOMES; BRITO, SAMANTHA CARVALHO MAIA; FREITAS-JUNIOR, LUCIO H.; et al. Nano-multilamellar lipid vesicles promote the induction of SARS-CoV-2 immune responses by a protein-based vaccine formulation. Nanomedicine-Nanotechnology Biology and Medicine, v. 45, p. 11-pg., 2022-08-31. (20/10700-3, 20/08943-5, 16/23560-0, 20/05204-7, 21/05661-1)

FARIAS, JESSICA PIRES; ANDREATA-SANTOS, ROBERT; BRITO, RUTH DALETY DA SILVA; SOUZA, MILENA SILVA; FOGACA, MAYANNA MOREIRA COSTA; PINHEIRO, JOSILENE RAMOS; DA CRUZ, EDGAR FERREIRA; LIANG, WILLIAN; SIMOES, RAFAEL DA CONCEICAO; LUIZ, WILSON BARROS; et al. The fourth COVID-19 vaccine dose increased the neutralizing antibody response against the SARS-CoV-2 Omicron (B.1.1.529) variant in a diverse Brazilian population. MICROBIOLOGY SPECTRUM, v. N/A, p. 16-pg., 2023-11-01. (20/05204-7, 21/05661-1, 20/08943-5, 22/11981-1)

CLARK, JORDAN J.; HOXIE, IRENE; ADELSBERG, DANIEL C.; SAPSE, IDEN A.; ANDREATA-SANTOS, ROBERT; YONG, JEREMY S.; AMANAT, FATIMA; TCHEOU, JOHNSTONE; RASKIN, ARIEL; SINGH, GAGANDEEP; et al. Protective effect and molecular mechanisms of human non-neutralizing cross-reactive spike antibodies elicited by SARS-CoV-2 mRNA vaccination. CELL REPORTS, v. 43, n. 11, p. 21-pg., 2024-11-05. (21/05661-1, 23/02345-7, 20/08943-5)