Molecular characterization of the in vivo effects of Bothrops jararaca venom: analysis of mouse muscle tissue and plasma, by proteomic and immunohistochemical approaches

• Grant number: 21/10570-5
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2021
• End date: March 31, 2026
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Solange Maria de Toledo Serrano
• Grantee: Bianca Carla Silva Campitelli de Barros
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling, AP.CEPID

Abstract

Envenoming by Viperid snakes is a critical public health problem in Brazil and is recognized by the World Health Organization (WHO) as one of the most important Neglected Tropical Diseases, causing a high percentage of permanent disability and, frequently, death. It is well established that systemic effects of envenomation by Bothrops snakes comprise hemorrhage, coagulopathy, shock, and renal failure. However, the molecular mechanisms involved in these effects are only partially known. On the other hand, it is recognized that these disorders are mainly promoted by Snake Venom Metalloproteinases (SVMP), Snake Venom Serine Proteinases (SVSP), phospholipases A2 (PLA2), and C-Type Lectins (CTL). In this scenario, mass spectrometry-based proteomic analysis of local tissue and blood plasma, and immunohistochemical analysis are powerful approaches to integrate the findings of local and systemic effects in the pathogenesis induced by snake venoms. The proposal of post-doctoral training is to molecularly characterize the effects of Bothrops jararaca venom on the thigh muscle and blood plasma, in a murine model, using integrative technologies of mass spectrometry and immunodetection of tissue damage. In addition, by the same approaches, the impact of the anti-bothropic antivenom treatment after the venom injection, will also be evaluated in this model. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ASEGA, AMANDA F.; BARROS, BIANCA C. S. C.; CHAVES, ALISON F. A.; OLIVEIRA, ANA K.; BERTHOLIM, LUCIANA; KITANO, EDUARDO S.; SERRANO, SOLANGE M. T.. Mouse skin peptidomic analysis of the hemorrhage induced by a snake venom metalloprotease. Amino Acids, v. 55, n. 9, p. 17-pg., 2023-06-30. (21/10570-5, 13/07467-1, 20/12317-2, 10/17328-0, 11/11308-0, 11/16623-1, 10/00206-0)