Analysis of flow, biochemical composition and salivary redox state of rats treated with the anticonvulsant Levetiracetam

• Grant number: 21/10604-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: December 01, 2021
• End date: November 30, 2022
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Antonio Hernandes Chaves Neto
• Grantee: Douglas Sadrac de Biagi Ferreira
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

Research shows evidence of the association of epilepsy and the use of anticonvulsants with the increased prevalence of oral health problems. Therefore, the objective of this research will be to analyze the possible effects of the chronic use of anticonvulsant levetiracetam (LEV) on the flow, biochemical composition and salivary redox state of rats. Therefore, 6-week-old male Wistar rats will be randomly divided into 3 groups (n=8/group): Control, LEV100 and LEV300. The treatment will take place for 21 consecutive days and the anticonvulsant in the form of syrup (Keppra®, UCB Biopharma Ltda, Limay, France) will be administered via intragastric gavage at doses of 100 mg/kg and 300 mg/kg, which is approximately equivalent to the therapeutic dose initial dose and maximum daily dose in humans, respectively, based on the formula for translational dose based on body surface area (mg/m²). The Control group will receive 0.9% NaCl (w/v) the volume equivalent to the maximum dose by the same route. After the experimental period, the animals will be anesthetized for the collection of saliva induced by pilocarpine and immediately, the flow, pH and buffering capacity will be determined. Analyzes of total protein content, salivary amylase activity, lipid oxidative damage by TBARs (thiobarbituric acid reactive substances), protein oxidative damage by carbonylation by reaction with 2,4-dinitrophenylhydrazine ( DNPH) and total non-enzymatic antioxidant capacity by the FRAP (ferric reducing - antioxidant power) method. Data normality and homoscedasticity will be analyzed to select the most appropriate statistical test. For all tests, the level of rejection of the null hypothesis will be set at 5% (p < 0.05). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FIAIS, GABRIELA ALICE; FERREIRA, DOUGLAS SADRAC DE BIAGI; FREITAS, RAYARA NOGUEIRA DE; SILVA, LUCAS GUILHERME LEITE DA; KAWAGUCHI, MARCELO; VERAS, ALLICE SANTOS CRUZ; TEIXEIRA, GIOVANA RAMPAZZO; ANTONIALI, CRISTINA; DORNELLES, RITA CASSIA MENEGATI; NAKAMUNE, ANA CLAUDIA DE MELO STEVANATO; et al. Assessment of the toxic effects of levetiracetam on biochemical, functional, and redox parameters of salivary glands in male Wistar rats. Toxicology, v. 496, p. 12-pg., 2023-09-01. (21/10604-7)