Developing broad-spectrum resistance to turnip mosaic virus in rapeseed

• Grant number: 21/11449-5
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• Start date: March 01, 2022
• End date: February 28, 2023
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Principal Investigator: Anete Pereira de Souza
• Grantee: Ricardo José Gonzaga Pimenta
• Supervisor: John A. Walsh
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Institution abroad: University of Warwick, England
• Associated to the scholarship: 19/21682-9 - Associative mapping and transcriptomic in the investigation of sugarcane yellow leaf virus resistance, BP.DD

Abstract

Rapeseed (Brassica napus) is the second most important oleiferous crop worldwide, and is also highly valuable as a polyploid model species. One of the most relevant pathogens infecting this crop is turnip mosaic virus (TuMV), which is transmitted by several aphid species. The most efficient resource to avoid yield losses associated to TuMV is host resistance, which has been identified in rapeseed and other Brassica species. However, sources of recessive resistance, which are particularly useful due to their broadness and durability, have not been found occurring naturally in B. napus. In this project, we propose to assess the level and spectrum of resistance to TuMV in several gamma ray-mutagenized rapeseed lines. We also intend to investigate the allele profile and expression pattern of eukaryotic initiation factor 4E (eIF4E/eIF(iso)4E) genes, previously identified by Prof. John Walsh's group as a source of recessive resistance to TuMV in the closely related Chinese cabbage (Brassica rapa). Understanding of the relationship between the genotypic composition and expression profile of eIF4E/eIF(iso)4E genes and rapeseed resistance to different TuMV isolates would not only bring substantial contributions to scientific knowledge, but also bears the potential to assist the release of new rapeseed cultivars with broad and durable resistance to TuMV. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MACLEOD, KYLE; GREER, SHANNON F.; BRAMHAM, LAWRENCE E.; PIMENTA, RICARDO J. G.; NELLIST, CHARLOTTE F.; HACKENBURG, DIETER; TEAKLE, GRAHAM R.; BARKER, GUY C.; WALSH, JOHN A.. A review of sources of resistance to turnip yellows virus (TuYV) in Brassica species. Annals of Applied Biology, v. N/A, p. 9-pg., 2023-08-15. (21/11449-5)