Evaluation of the effects of high fat diet and physical exercise on the expression of Parp1 and the protein parylation profile in the hypothalamic tissue

• Grant number: 21/11812-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: November 01, 2021
• End date: October 31, 2022
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Eduardo Rochete Ropelle
• Grantee: Matheus Biscaro Rocha
• Host Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil
• Associated research grant: 19/21709-4 - Implications of mitonuclear imbalance and UPRmt in hypothalamic neurons in the genesis of Obesity, AP.TEM

Abstract

Mitochondria present in hypothalamic neurons are essential for the control of energy homeostasis. The consumption of diets rich in saturated fats can promote changes in the functioning of these organelles in some subpopulations of hypothalamic neurons, promoting changes in the signals that control satiety and body energy expenditure, contributing to the development of the obese phenotype. The intracellular bioavailability of NAD+ has been shown to be a determining factor for the maintenance of mitochondrial activity in several cell types. In cases of inflammation, accumulation of reactive oxygen species or DNA damage, NAD+ levels can be reduced to critical levels, compromising mitochondrial morphology, dynamics, and activity. In this context, poly [ADP-ribose] polymerase-1 (PARP1) can be considered one of the main consuming enzymes of NAD+ in situations of cellular stress. On the other hand, exercise is known for its anti-inflammatory, anti-oxidant actions, in addition to stimulating biosynthesis and mitochondrial function. However, the effect of a saturated diet and exercise on PARP1 content in the hypothalamus has not yet been determined. Therefore, the aim of this project will be to evaluate the protein content of PARP1 and the levels of protein parylation in the hypothalamic tissue of lean, obese, and exercised obese mice. The realization of the current project may contribute to the elucidation of the activity of hypothalamic PARP1 in response to the consumption of a high-fat diet and physical exercise.(AU)