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Effect of endodontic irrigants on bulk-fill resin-dentin interface with universal adhesive system

Grant number: 21/05476-0
Support type:Scholarships in Brazil - Master
Effective date (Start): April 01, 2022
Effective date (End): September 30, 2023
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal researcher:Ticiane Cestari Fagundes
Grantee:Érika Mayumi Omoto
Home Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

The success of endodontic treatment is related to the interface quality of the restorative material and the dental substrate, ensuring a coronal sealing. It is recommended to perform the dental restoration immediately after the endodontic treatment, although some irrigants have deleterious effects on dentin tissue, which can negatively affect the adhesion of restorative materials. The objective of the study will be to evaluate the effect of endodontic irrigants on adhesion and sealing between bulk-fill resin and dentin using a universal adhesive system. Three experimental factors will be investigated: (I) irrigation solutions at two levels: 2% chlorhexidine and 5.25% sodium hypochlorite; (II) restorative systems at three levels: a conventional adhesive and composite resin system and two universal adhesive systems and two bulk-fill resins; (III) depth of restorations at three levels: coronary third, middle third and cervical third. Sixty six human molars will have the pulp chamber roof, pulp and roots removed and will be divided into 6 groups according to the type of endodontic irrigant and the restorative system used: G1 - 2% chlorhexidine + conventional adhesive system (Adper Single Bond 2 ) + conventional composite resin (Filtek Z350 XT); G2 - 2% chlorhexidine + universal adhesive system (Single Bond Universal) + bulk-fill composite resin (Filtek One Bulk-fill); G3 - 2% chlorhexidine + universal adhesive system (Tetric N-Bond Universal) + bulk-fill composite resin (Tetric N-Ceram Bulk Fill); G4 - sodium hypochlorite 5.25% + conventional adhesive system (Adper Single Bond 2) + conventional composite resin (Filtek Z350 XT); G5 - sodium hypochlorite 5.25% + universal adhesive system (Single Bond Universal) + bulk-fill composite resin (Filtek One Bulk-fill); G6 - 5.25% sodium hypochlorite + universal adhesive system (Tetric N-Bond Universal) + bulk-fill composite resin (Tetric N-Ceram Bulk Fill). Cross sections will be made with a diamond disc in order to obtain two rectangular sticks (0.9 ± 0.1 mm2) from each section (coronary, middle and cervical thirds), performing six sticks per tooth. After 24 hours, half of the sticks will be subjected to microtensile (n=8 per group) and nanoleakage (n=3 per group) tests. The other half will be subjected to thermocycling for further analysis. If the data obtained pass statistical tests of normality and homogeneity, it will be analyzed with three-way repeated measures ANOVA and Tukey test, with a significance level of 5%. (AU)

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