Collagen and silk fibroin patches with platelet-rich plasma (PRP) to post-ischemic myocardium remodeling and regeneration: experimental study in rats

• Grant number: 21/14160-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2022
• End date: March 31, 2023
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Lindemberg da Mota Silveira Filho
• Grantee: Rachel Janeiro Antonio
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Ischemic heart disease is the most common chronic condition worldwide. Biomaterial patch implant into the infarcted epicardial surface has been applied as a possible treatment. Usual therapies result in a wide range of patients with post-ischemic heart failure, triggered by unfavorable ventricular remodeling. Moreover, the application of biodegradable scaffolds associated with platelet-rich plasma (PRP), may offer mechanical support to the left ventricle, and influence the structural microenvironment with cardiac remodeling and quimiotaxis of new cells. This study aims to evaluate the application of biodegradable scaffold patches applied over the infarcted area, with and without the association of PRP. Rats will undergo myocardial infarction. After two weeks, biocompatible, biodegradable and bioabsorbable patches made of collagen and silk fibroin seeded with PRP will be sutured to infarcted myocardium and compared to the same biomaterial without PRP and MI control. The animals will undergo baseline echocardiography, before patch implant and then at 2, 4 and 8 weeks after treatments. Eight weeks after treatment, they will be euthanized, and hearts will be harvested. The hearts will be analyzed on Langendorff apparatus and subsequently submitted to histology and measurement of the proportional area of infarction and ventricular thickness. Samples will be isolated for TNF and IL6 processing by Western blotting. We hope to find echocardiographic outcomes related to better left ventricular remodeling and smaller areas of infarction in treated groups and eventually differences in the effects of scaffolds with the application of PRP. If these results are achieved, this will reinforce the possibility of translational approaches applying these biomaterials in similar clinical scenarios in the future. (AU)