Effect of photo-ozone therapy on Candida monospecies biofilms and in the viability of human keratinocyte cell line HaCaT

• Grant number: 22/00678-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: July 01, 2022
• End date: December 31, 2022
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Jair Camargo Ferreira
• Grantee: Gabriella Rocha Vicente de Souza
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

Biofilms are microbial communities embedded in extracellular matrix with high adhesion power and extreme antimicrobial resistance. Study performed by our research group showed the germicidal action of photo-ozone therapy against oomycetes (FAPESP 2020/14324-6). The goals of the present study will be to characterize the antibiofilm action of photo-ozone therapy and its effect on the viability of keratinocytes. Biofilms of Candida (C. albicans, C. tropicalis and C. parapsilosis) will be treated with low-level laser (LLLT), ozone gas (O3) or photo-ozone therapy (LLLT-O3). In the LLLT group, the biofilms will be irradiated for 160 seconds with an iodine laser with a light absorption wavelength of 660nm and a final fluence of 80 J cm2. In the O3 group, the biofilms will be exposed to an O2-O3 gas mixture containing 50 ¼g O3 mL-1 for 15 min. Biofilms from the LLLT-O3 group submitted to LLLT and O3 treatments in sequence. The effect of treatments (LLLT, O3 and LLLT-O3) on cell viability will be determined through their cytotoxicity and antiprophylactic potential on human keratinocytes (HaCaT). Positive and negative controls will be included in the antimicrobial and the cell viability analyses. The results will support future research on the antimicrobial and regenerative efficacy of photo-ozone therapy using in vivo models.(AU)