Evaluation of 6-nitronoradrenaline in rat vas deferens

• Grant number: 22/06644-6
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2022
• End date: May 31, 2023
• Field of knowledge: Biological Sciences - Pharmacology - Cardiorenal Pharmacology
• Principal Investigator: Gilberto de Nucci
• Grantee: Luiza Pires Ferreira Novaes da Silva
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 19/16805-4 - Evaluation of the pathophysiological role of endothelial catecholamines, AP.TEM

Abstract

Electric field stimulation (EFS) is a technique in which a stimulus of Electricity is applied uniformly to a tissue insulated in pulses of waves short. Depending on the mediators released by the SAI, contraction or relaxation of tissues. Initially, our research group demonstrated that stimulated contractions of umbilical cord vessels (HUCV) depend on the presence of the endothelium and are not affected by the tetrodotoxin, a sodium channel blocker, used to prevent the release of mediators of nerve endings. Similar findings were found in reptiles. the aorta of the tortoise Chelonoidis carbonaria and aorta of Crotalus durissusterrificus, Bothrops jararaca, and Panterophis guttatus. Recently, it was detected by pharmacological assays and by liquid chromatography coupled to tandem mass spectrometry (LC-MS-MS) the basal release of dopamine by HUCV endothelial cells. Dopamine acts on dopaminergic neurotransmission in the central nervous system, in the modulation of blood pressure by renal and vascular mechanisms, and is important in the modulation of cardiac function. LC-MS-MS analyses also revealed a new vascular mediator 6-nitrodopamine. 6-nitrodopamine is produced from dopamine nitration. In studies, our research group identified the release of 6-nitrodopamine in the vas deferens in rats this work aims to characterize the action of 6-nitrodopamine on beta-adrenergic receptors in the rat vas deferens duct.(AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BRITTO-JUNIOR, JOSE; LIMA, ANTONIO TIAGO; CAMPOS, RAFAEL; GIAO, ALEXANDRE ASTURIANO; MENDES, GUSTAVO DUARTE; SILVA, LUIZA P. FERREIRA-NOVAES P.; FREGONESI, ADRIANO; PUPO, ANDRE SAMPAIO; ANTUNES, EDSON; DE NUCCI, GILBERTO. 6-Nitrodopamine potentiates contractions of rat isolated vas deferens induced by noradrenaline, adrenaline, dopamine and electric field stimulation. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, v. N/A, p. 16-pg., 2023-04-04. (21/14414-8, 17/15175-1, 19/16805-4, 22/08232-7, 22/06644-6, 21/13593-6)