Comparative genomics of bacteriocin-related genes from Ligilactobacillus salivarius

• Grant number: 22/10612-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2022
• Effective date (End): July 31, 2023
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Alessandro de Mello Varani
• Grantee: João Victor dos Anjos Almeida
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated research grant: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems, AP.TEM

Abstract

The use of bacteriocins is a promising, efficient, and current approach to food conservation, as it aims to reduce pathogenic microorganisms that cause economic losses in poultry and swine farming. Thus, this proposal intends to identify and understand possible relationships between bacteriocins, their sequences, and the niche to obtain by genomic and data mining studies focusing on bacteriocin-producing genes in Ligilactobacillus salivarius of agricultural interest. For this, 398 genomes of Ligiactobacillus salivarius from the "Genomes - NCBI Datasets" platform will be evaluated, and the prediction and typing of bacteriocins will be performed using the BAGEL4 tool. Then, the sequences will be manually curated through MAFFT alignment, visualization by the CLC Genomic Viewer, and phylogenetic analysis by maximum likelihood. Additionally, mobile genetic elements will be analyzed by PHASTER and the Mobile Element Finder and 3D structure prediction by the ROBETTA platform. It is expected that the analysis of the results obtained will contribute to a greater genomic understanding of these metabolic products, increasing not only literary knowledge but their possible application in the production chain.(AU)