Development of imunosensor based on core-shell formed by ZnOAu to determine antidoby anti-asparaginase on patients with leukemia

• Grant number: 22/09900-3
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: October 01, 2022
• End date: September 30, 2023
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Renata Kelly Mendes Valente
• Grantee: Bruna Bragantin
• Host Institution: Escola Politécnica (EP). Pontifícia Universidade Católica de Campinas (PUC-CAMP). Campinas , SP, Brazil

Abstract

Acute Lymphoblastic Leukemia (ALL) is the most common type of cancer in children and adolescents, which affects lymphoid cells and, if left untreated, can worsen quickly. However, an effective treatment has a rate of up to 90% ofcure. Currently, the administration of the enzyme asparaginase is chemotherapy most used in these cases. This enzyme consumes extracellular asparagine, which is essential for the development and maintenance of the tumor cell, producing L-aspartate and ammonia. However, as it is an enzyme not human, the organism of some patients can produce anti-asparaginase antibodies, which cause enzymatic inactivation, impairing the treatment efficiency. Thus, the quantification of these antibodies is essential for the medical team can make the best decision regarding chemotherapyantitumor. The methods used for the anti asparaginase determination are complex and laborious. A simpler and lower cost alternative refers to use of electrochemical biosensors, which presents high sensitivity and selectivity. However, in order to present these advantages, it is necessary that the biorecognition element is strongly immobilized on electrode surface. In this sense, the use of core-shell has shown great interest in recent years and, although with few studies, presents good prospects as a platform for biomolecules immobilization. The core-shell, in this case, combines the properties ZnO nanoparticles (core) with those of gold nanoparticles (shell), producing a nanostructure with unique advantages. In this context, this project aims to develop a biosensor (immunosensor) based on asparaginase immobilized on ZnO@Au core-shell for anti-asparaginase determination in real samples.