Consequences and adverse effects of exposure to a mixture of endocrine disrupting agents on the lungs in rats

• Grant number: 22/13812-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2023
• End date: December 31, 2023
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Renata Calciolari Rossi
• Grantee: João Pedro Dearo Regueiro
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil

Abstract

The evolution of industrial capacity brought with it a shower of consequences derived from the manipulation and release into the environment of toxic and oncogenic materials, which when in contact with individuals, cause physiological and metabolic consequences, being in rural areas or mainly in urban areas, decreasing the life expectancy of exposed individuals. Xenobiotics are components considered foreign to the human body and among them are Endocrine Disruptors (EDs) that are substances found in the environment in small concentrations, but enough to cause adverse effects to animals and humans exposed in the long term. The lungs have a very important communication with the external environment through the air - which has the potential to carry thousands and different types of toxins and even microorganisms to the pulmonary epithelium, leading to the emergence of various adverse effects on respiratory health - thus - the lungs EDs have the ability to directly affect this vital organ. Despite the large number of studies present in the literature regarding ND, there is no study that performs analysis of the pulmonary epithelium using the reading and research of morphometric and histopathological alterations of this tissue, relating the effects, consequences and pulmonary alterations as the present study aims to explore. The objective of this work is to evaluate the consequences and adverse effects on the lungs, as a result of exposure to a mixture of Endocrine Disruptors, in rats exposed to Endocrine Disruptors. Thus, a research project was carried out regarding aspects of the physiology-environment relationship in exposure to a mixture of endocrine disruptors using rats for the exposure of EDs and assessment of their toxicity. For this, a mixture of twelve chemical compounds selected as representatives of other DEs was used, simulating an exposure to the toxic substance by the human being. The composition of the mixture consists of 8 compounds characterized by having predominantly androgenic inhibitory properties added to the presence of four substances with xenoestrogenic characteristics. For the statistical analysis, the Sigma Stat® version 2.0 will be used, and the cases in which the data present normal distributions will be analyzed using parametric tests for two groups such as t of student. Otherwise, non-parametric tests such as the Mann-Whitney test will be used. Differences will be considered statistically significant when p is less than 5% (p<0.05).