Training in cell culture, single cell sequencing and related techniques with adipocytes

• Grant number: 22/10182-8
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• Start date: May 08, 2023
• End date: September 07, 2023
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: João Paulo Gabriel Camporez
• Grantee: Guilherme Fancio Bovolin
• Supervisor: Evan Rosen
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Harvard University, Boston, United States
• Associated to the scholarship: 22/00231-1 - Estradiol effects on white adipose tissue browning in female mice, BP.IC

Abstract

Adipose tissue was once considered only as a storage energy organ and protect the organism from the cold exposure.However, it is now clear that adipose tissue is a metabolic active organ that regulates energy homeostasis, insulin sensitivity, thermogenesis and also is recognized by its plasticity and dynamicity. Our research in Brazil uses ovariectomized mice models to investigate the estradiol effects on browning in the adipose tissue. The inductance of this process has been investigated because increasing the brown adipose tissue activity and also browning of the white are associated with an improvement in metabolic health and also can become therapeutic targets for weight control. Thus, we are interested in understanding how estradiol interacts with fat cells and influences the adipogenesis of brown and beige adipocytes, once less is known about the mechanism involved in the estradiol effects on the browning. Conversely, the laboratory of Rosen aim is to understand the transcriptional pathways that underlie metabolic diseases and have longstanding interest in using genomic and epigenomic approaches to identify novel transcriptional factors and pathways that regulate processes such as adipogenesis and lipid handling, insulin resistance and metabolic memory. Therefore, the objective of this internship is to improve the understanding of adipocyte biology and learn techniques such as cell culture, single cell sequencing, metabolic phenotyping and related experiments. In that regard, the implication of this project will provide advancement in the development of our research in Brazil. (AU)