Development of mucoadhesives containing antifungal agents complexed with beta-cyclodextrin: evaluation of the anti-inflammatory potential and cytocompatibility

• Grant number: 23/00292-3
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: June 01, 2023
• End date: December 05, 2023
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Karin Hermana Neppelenbroek
• Grantee: Carolina Yoshi Campos Sugio
• Supervisor: Maria Helena Raposo Fernandes
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Institution abroad: Universidade do Porto (UP), Portugal
• Associated to the scholarship: 21/15142-1 - Mucoadhesives containing antifungal complexed with beta-cyclodextrin for Denture Stomatitis treatment, BP.DR

Abstract

Denture stomatitis (DE) is the most prevalent oral candidiasis among users of removable dentures. Although conventional treatment with topical or systemic antifungals is effective in relieving signs and symptoms, reinfection of the oral mucosa has been commonly observed within two weeks after treatment. In recent decades, mucoadhesives with a drug delivery system have become a trend in pharmaceutical technology, as they enable faster absorption directly at the infected site, in addition to better adhesion in mucosal tissues and drug bioavailability. Furthermore, inclusion complexes with cyclodextrins have been suggested to increase drug solubility and molecule stability. Preliminary results of the mucoadhesive formulation demonstrated satisfactory bioadhesive and antifungal capabilities. Therefore, the aim of this study will be to evaluate the anti-inflammatory potential and cytocompatibility of mucoadhesives containing antifungals (nystatin or chlorhexidine) complexed or not to ²-cyclodextrin, for the treatment of PE. Using the indirect method of quantification of nitric oxide by macrophages, the anti-inflammatory potential and cytocompatibility in L929 fibroblasts will be evaluated through resazurin reduction assays, lactate dehydrogenase release, mitochondria and F-actin cytoskeleton tracking, of apoptosis by flow cytometry and production of reactive oxygen species (ROS). Data will be obtained and subjected to appropriate statistical tests. (AU)